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As a new energy , the development of fuel ethanol , used to alleviate the energy crisis and improve the situation of environmental pollution are of great significance . Consider to reduce the production of fuel ethanol cost perspective , the use of a cellulose - based material as raw material, is undoubtedly the best choice . Therefore, the efficiency of cellulose degradation strains is key to the application of the technology . Thermophilic side Neurospora crassa (Sporotrichum thermophile) is a thermophilic filamentous fungi , break down cellulose characteristics . Morphology of solid PDA culture conditions , thermophilic side cinerea strains branched mycelium plexus , separated conidia light brown , apical or lateral . ITS sequence molecular classification found thermophilic side Neurospora thermophilic the leather section spore ( Scytalidiumthermophilium ) and specific Humicola ( Humicola insolens ) thermophilic bacteria away . Reducing sugar content and bagasse reduction of indicators , liquid fermented bagasse as sole carbon source , indicating that in the the bagasse 30g / L , the NH 4 < / sub > NO 3 < / sub > 11.45 g / L, initial pH 4.0, 15% of the inoculum size ( spore suspension concentration 10 6 / mL) conditions , bagasse degradation effect . To the thermophilic laterosporus mold ATCC 36347 strain as the starting strain , sodium carboxymethyl cellulose - Congo red medium by UV nitrite compound mutation screening , 12 transparent ring strains . According to the size of the transparent circle diameter ratio of the diameter of colonies represent the enzyme activity , analysis of variance, the initial determination of endoglucanase activity was the highest strain 66-22 . 66-22 strain optimal culture conditions : 45 ° C , NH4NO3 1.5g / L , initial pH of 6.0 . Under the above conditions , the endoglucanase activity to the highest in 2 and 3 Telsda four days began to decrease , 2 and 3 days of enzyme activity was not significantly different . Were cultured under optimal culture conditions , enzyme activity in 2d and 3d ,66 -22 strains were thermophilic side Neurospora crassa ATCC 36347 1.19 times and 1.29 times .
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