Dissertation > Excellent graduate degree dissertation topics show

Cloning and Expression of the Region A Gene of Clumping Factor A of Staphylococcus Aureus from Bovine Mastitis

Author: JiangXiaoJuan
Tutor: HaoYongQing
School: Inner Mongolia Agricultural University
Course: Preventive Veterinary Medicine
Keywords: Mastitis in dairy cattle Staphylococcus aureus Clumping factor A (ClfA) Clone Expression
CLC: S852.61
Type: Master's thesis
Year: 2008
Downloads: 84
Quote: 1
Read: Download Dissertation


Mastitis cows a common and frequently occurring, the main reason for its occurrence is infected with pathogenic microorganisms. Causing mastitis as many as 80 kinds of pathogenic microorganisms, including bacteria, mycoplasma, fungi and viruses. Staphylococcus aureus dominant bacterial pathogen microorganisms, but over the years there has been no effective measures for the prevention and treatment of Staphylococcus aureus mastitis This paper Staphylococcus aureus cohesion factor AA District gene cloning expression for the developed prevention of Staphylococcus aureus mastitis bioengineering vaccine to provide a basis. ClfA A zone of the GeneBank published NewMan strain primers were designed, the test genotypes were amplified using the PCR method Hohhot surrounding areas exist four major Staphylococcus aureus, Staphylococcus aureus clumping factor A ( ClfA) A region genes. Experiments on four different genotypes of Staphylococcus aureus clumping factor A (ClfA) A region genes were cloned and sequenced, with Genbank standard landing NewMan of strain ClfA A region sequences than genotype 3, type 9 gene, gene 11 the type and NewMan strains homologous, respectively, 97.8%, 96.5%, 98.3%, 85.4% the gene 5 Newman strains homology only. Type 3 gene, gene type 9, type 11 gene between the homology is higher, and gene 5 and three additional genotype homology also lower. The measured sequences the use DNAstar software epitopes analysis, genotype 3 than NewMan strains, type 9 gene in an antigenic sites, selected a genotype that gene 9 type expression. The fragment was cloned into the prokaryotic expression vector pET-32a (), to obtain the recombinant plasmid pET-ClfA-A region. Be transformed into E. coli BL21 (DE3) induced by IPTG, expressed ClfA-A District proteins. Positioning analysis, the target protein exists in a soluble form. His · bind pillar after purification to obtain high purity ClfA-A region protein. The purified protein and Freund's adjuvant immunizing rabbits by three after immunization, high titer antiserum. Anti-adhesion capacity detected by ELISA, tube agglutination test, antibody, engulfed the conditioning test prove that the purpose of protein adhesion activity, homemade hyperimmune serum against Staphylococcus aureus adhesion to the role of bovine fibrinogen swallowed opsonization. The experiments confirmed that the expressed protein to the ClfA-A region protein.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. The Research of the High-Rise Building’s Form and Expression in the New Century,TU971
  3. Research on Design of Teaching Buildings for Arts Departments of Higher Learning Institutions,TU244.3
  4. Research on Temporal Information Recognition and Normalization,TP391.1
  5. Expression of D-AtCGS in E. Coli and Preparation of Polyclonal Antibody Against D-AtCGS,Q943.2
  6. The Study on Arabidopsis Thaliana Heat Shock Factor HSFA 1d Response to Formaldehyde Stress,Q945.78
  7. Expression of hBMP4 and hBMP7 in Chinese Hamster Ovary Cells,Q78
  8. Cloning and Expression Analysis of GPx, GST and SAHH Genes in Chlamydomonas Sp. ICE-L from Antarctica,Q943.2
  9. cDNA Cloning, Expression of vp5 and vp7 Genes and Subcecullar Localization of VP5 and VP7 Proteins in Grass Carp Reovirus,S941.41
  10. Cloning, Expression of vp6 and ns38 Genes and Immunogenicity of VP6 and NS38 in Grass Carp Reovirus,S941.41
  11. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  12. The Expression Survey of BMP Signalling Pathway in the Human Embryonic Tooth Germ,R78
  13. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  14. Cloning and Expression of Growth Hormone Gene in Cobia (Rachycentron Canadus),Q786
  15. Cloning and Characterization of BAFF Gene from Whitespotted Catshark and Crucian Crap,S917.4
  16. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  17. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  18. Comparative Study on Reproductive Biological Characteristics of Helicoverpa Armigera and Helicoverpa Assulta (Lepidoptera:Nuctuidae),S433
  19. Cloning, Expression and Polymorphism Analysis of BMP4 Gene in Hu Sheep,S826
  20. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  21. Cloning and Expression Analysis of Scavenger Receptor Class B Type Ⅰ and Antifreeze Proteinstype Ⅱ Genes in Lutjanus Sanguineus,S917.4

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Pathogenic bacteria
© 2012 www.DissertationTopic.Net  Mobile