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Cytology and Technology of Chromosome Doubling of Alnus Cremastogyne Burk and A.formosana

Author: YangWeiGuang
Tutor: KangXiangYang
School: Beijing Forestry University
Course: Tree Genetics and Breeding
Keywords: Sichuan alder Taiwan alder Meiosis Colchicine Pollen chromosome doubling
CLC: S792.14
Type: Master's thesis
Year: 2008
Downloads: 93
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Abstract


Sichuan alder (Alnus cremastogyne Burk) and Taiwan the alder (A.formosana) are Betulaceae Alnus (Betulaceae) plants (Alnus), less related to genetic improvement research, seed degree low. In order to meet the urgent needs of our short-cycle industrial timber construction varieties, Sichuan Alder and Alder material on the Sichuan alder and of Taiwan alder pollen mother cells meiosis Megasporogenesis embryo sac development, and 2n pollen induction technology and other research, the results of the study are as follows: (1) Sichuan alder pollen mother cells meiosis approximately in late February start, from a thin line to pre-tetrad meiosis takes about 9 ~ 18d after ; alder pollen mother cells meiosis around late September started from the early to the thin line tetrad meiosis is completed takes about 7 ~~ 12d; process of pollen mother cells exist are not synchronized, single the PMC meiosis process differences between the strains by the interaction of genetic and environmental, of which Sichuan alder and development, the fastest and the slowest single plant pollen mother cells process almost 13d Taiwan alder almost 20d . Sichuan alder, the process of Taiwan alder pollen mother cells with male inflorescence the external morphology changes exist certain related flower bud morphological according to the flower bud length, diameter and color, the ability to distinguish the pollen mother cell meiosis process. (2) treatment period, the colchicine solution concentration and the processing time of Sichuan alder 2n pollen yield differences significant 0.8% colchicine solution in pachytene continued treatment 36h 2n pollen was the highest rate of 41.61% ; treatment period, the of colchicine solution concentration and processing time of the alder pollen yield differences are not significant, with 0.5% colchicine solution in the the pachytene continued processing 48h maximum 2n pollen yield was 13.50%, The reason may be less than the amount of applied treatment of colchicine solution. (3) the alder Size spores occur out of sync, pollen mother cells start as early as the megaspore mother cell meiosis; megaspore mother cells and pollen mother cells in meiosis process there is a certain timing correlation and megaspore meiosis process of embryo sac development and female inflorescence external morphological changes more closely, according to these two points largely determine the female inflorescence which megasporocyte during meiosis and embryo sac development period, Taiwan alder the female gametes chromosome doubling timely basis.

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CLC: > Agricultural Sciences > Forestry > Forest tree species > Broad-leaved trees > Alder
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