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Study on the Breakway of Sapstain Fungi and Selection of Biological Controlling Fungi and Study on the Antagonism of Biological Controlling Fungi to Sapstain Fungi

Author: ZhangYu
Tutor: XuFeng
School: Guangxi University
Course: Forest cultivation
Keywords: Sapstain fungi Trichoderma sp. Ientification Bological controlling Atagonism
CLC: S782.33
Type: Master's thesis
Year: 2008
Downloads: 87
Quote: 1
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Abstract


In this test,we got 2 sapstain fungi and 6 white fungi from Hevea brasiliensis and Pinus massoniana in the first selection.And they are identified by the way of morphdosy identification and molecular identification.The sapstain fungi are Sphaeropsis sapinea and Botryosphaeria rhodina.The white fungi are Trichoderma atroviride, Trichoderma citrinoviride,Trichoderma aureoviride,Trichoderma koningii,Trichoderma koningiopsis and Trichoderma asperellum.Through studies of fungi’s biology characteristics,the two sapstain fungi can fit in with large temperature confines.The best suitable temperature for Trichoderma citrinoviride is between 30℃to 40℃.And the others are between 20℃to 30℃.The two sapstain fungi are growth well under sunlight and it can fit in with a wide amphotericity.Six Trichoderma fungi like the acidic environment.The two sapstain fungi and white fungi are all need different carbon,but the peptone can help them growth well.There are two Trichoderma.sp.fungus which have capability of controlling to sap sapstain on the culture medium of PDA.Trichoderma koningii is the best one among them,which can control sapstain fungus Botryosphaeria rhodina and Sphaeropsis sapinea.Trichoderma koningiopsis、Trichoderma atroviride、Trichoderma asperellum、Trichoderma citrinoviride and Trichoderma aureoviride behind it. Although trichoderma citrinoviride cannot control the sapstain fungi under normal temperature,under temperature 30℃~40℃,it grow rapidly,produce a lot of conidia,fight nutritious dimension quickly and has apparently antagonism to sapstain fungi.Especially put it under 40℃.Put the colony of Trichoderma koningii and Trichoderma aureoviride spores on woods by spraying,the woods are not polluted by sapstain after a week.It is demonstrate that Trichoderma koningii and Trichoderma aureoviride are nice biological control fungus which are effective.

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CLC: > Agricultural Sciences > Forestry > Forest harvesting and utilization > Timber mining transport to school > Storage timber and custody > Wood Preservation
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