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Studies on Community Characteristics and Propagation Technique of the Endangered Plant Parakmeria Omeiensis Cheng

Author: ChenYing
Tutor: MaMingDong
School: Sichuan Agricultural University
Course: Forest cultivation
Keywords: Emei Parakmeria Community Characteristics Tissue culture PPO activity Total phenol content
CLC: S792.99
Type: Master's thesis
Year: 2008
Downloads: 156
Quote: 2
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Abstract

Emei Parakmeria (Parakmeria omeiensis Cheng) is endemic in China, located in Sichuan Emeishan wild plants only remaining twenty several strains, at the national level and endangered plants. The study the characteristics of The Emei Parakmeria community, tissue culture, grafting technology, polyphenol oxidase (PPO) activity and total phenolic content. The results are as follows: 1. Emei to be single-sex Mulan community characteristics the Emei Parakmeria scattered in Emeishan toon the floor Biandantang rock evergreen broad-leaved forest plant species 50 species belonging to 48 genera and 37 families, which dicotyledons accounted absolute advantage, accounting for 80% of the whole community, families, containing more than three genera as dominant families, respectively, 10.8% of the Magnoliaceae, Fagaceae, Lauraceae, Rosaceae, total family number. The flora of the communities in tropical and subtropical nature, can be divided into nine types, the tropical plant ingredients distribution share is higher than the temperate zone plant ingredients, Pan-tropical the tropical distribution plant ingredients again mainly . Communities phanerophytes accounted for 74% of the number of species, which accounts for about 64%, the in phanerophytes and dwarf phanerophytes; chamaephyte 4%, 6% Hemicryptophytes, hidden bud plants and annuals in both 8%. Tree layer canopy density of 70% the important values ??higher Theaceae, Podocarpaceae, Fagaceae, Magnoliaceae plants, followed by Aceraceae and Lauraceae. Emei were scattered in communities Parakmeria average plant height of 20m, average about branches under high 13.8m diameter of 31cm, average crown 6m; regeneration layer a large number of wheat leaves Podocarpus, Wushan new wood sericea, Taxus, Wushan Neolitsea dominant in the layer no the Emei Parakmeria seedlings; shrub layer, the average coverage of 72%, the western Hubei Yushania dominant, followed spinosa Myrsine the sarsaparilla and southwest; single herb layer species coverage of 5% to 30%, mainly for the green green moss grass. All levels of species diversity index were calculated to draw the highest biodiversity of tree layer, Simpson index of 0.8610, the Shannon-Weiner index 2.3062, uniformity 0.8508, the lowest ecological dominance of 0.1390, followed by the regeneration layer 0.1823, the ecology of shrub layer the highest dominance 0.8521 uniformity minimum 0.1578. the Emei intends to single-sex Mulan tissue culture tissue culture materials best time is from April to May, the average contamination rate below 20%, and mercuric chloride stem segment explants best treatment (7 8) min pollution rate is low at this time, and axillary bud induction is not suppressed, the induction rate of 74.3%; added to the medium, 10 mg / L of VC and 1g / L of the PVP can be effectively controlled brown , 10mg / L VC effect best browning rate only controls 1/2, terminal buds start rate of 77.8%; favor Emei intends to single-sex Mulan stems with buds axillary bud induction medium formula combination: B5 6-BA 1.5 mg / L IBA0.1 mg / L, induction rate was 92.9%; suitable for axillary bud stem segment elongation growth medium: MS 6-BA1.0 IBA0.1 NAA0.1 or B5 6-BA1.5 mg / L IBA0.1 mg / L; favor a combination of the best the terminal bud startup culture medium formula: MS BA 0.5 mg / L IBA 0.05 mg / L; suitable Emei Parakmeria stems Inducing Callus best medium combination: B5 6-BA1.5 mg / L NAA0.5mg / L, the culture conditions for the dark culture; April drawn the best time for the induction of callus; MS (B5) 6-BA0.2mg / L ZT 0.2mg / L NAA0.1mg / L is suitable for shoot proliferation culture medium combination. Emei intends grafting survival rates between the different rootstocks of Mulan grafting techniques compare results for: the Henan Magnoliae> Magnolia> Jiangyou Kobushi different grafting season survival rate comparison: spring> autumn. 4 Emei Emei Parakmeria fresh stem segments of material Determination intends to Mulan PPO activity and total phenol content the PPO activity best experimental conditions and the nature of the research aimed to further explore Emei Parakmeria organizations cultivate the Browning Mechanism in the process to provide a reliable test methods and to provide a scientific basis for browning control. The test results found that the proposed Mulan PPO activity determination Emei optimum pH value of 7.0, the optimum temperature of 25 ° C, or high or low will reduce the activity of the pyrocatechol concentration of 0.14 mol / L PPO activity the VC, citric acid and Na ₂ SO ₄ three kinds inhibitor, the inhibitory effect of VC best; Emei intends a single magnolia PPO activity and total phenol content material climate of a relatively close relationship, higher growing season PPO activity than the dormant season, while growth strong season and dormant season total phenol content higher than pumping a little season; research group training early generation of stem segment explants PPO activity and total phenolic content dynamically PPO activity of one or two weeks after vaccination upward trend the changes come two weeks after declining total phenol content was increased by a decline in a rising trend.

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