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Functional Analysis of MYB Family Transcription Factors OsMYB2 in Rice (Oryza Sativa L)

Author: ZhangYang
Tutor: ChenJianMin；YaoQuanHong
School: Yangzhou University
Course: Cell Biology
Keywords: Rice MYB transcription factor Abiotic stress response Yeast in vivo binding
CLC: S511
Type: Master's thesis
Year: 2008
Downloads: 427
Quote: 2
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Abstract


Transcriptional regulation of plant growth and development, stress responses, signal transduction, disease resistance and a series of the most important forms of regulation of gene expression, the transcription factor is an important factor involved in the regulation of the level of gene transcription process. MYB transcription factor is one of the largest family of plant transcription factor, can be combined with cis-acting element in the MYB gene promoter-specific identification regulation biotic or abiotic stress-related series of gene expression. Rice transcription factor OsMYB2 MYB family of proteins known in the art in the amino acid sequence with other plants of the OsMYB2 cDNA comparison results show that the transcription factor has a very conservative R2R3MYB of domains. Sequence analysis showed OsMYB2 conserved domains in the N-terminal repeat contains two incomplete tryptophan, tryptophan repeat region forming a spiral - rotation - helix DNA binding domain, further comprising a potential ATP-GTP binding site, a pair of redox sensitive cysteine, and a nuclear localization sequence. Process on rice various stresses of processing and extraction of different organizations RNA be reverse transcribed to cDNA for RT-PCR analysis. The result is OsMYB2 treatment induced by drought (PEG), salt (200 mM NaCl) and ABA (200μM), but not cold-inducible. Tissue specific detection, OsMYB2 in roots, stems, leaves, flowering period spike, the Filling Stage spike have expression, but the highest level of transcription in the root. To better understand the the physiological functions rice OsMYB2 gene cloned and response to abiotic stresses relationship building the OsMYB2 gene plant expression vector, spend transformed Arabidopsis by Agrobacterium dipped after hygromycin, GUS activity was detected verified and PCR screened positive transgenic plants. The physiological function analysis showed that, compared with wild-type and transgenic lines to ABA performance surplus sensitive reaction of adversity performance is weaker, but more sugar reaction performance. The same time, the of OsMYB2 may regulate the downstream gene expression profiling results RD22 and RD29A expression is down, ADH expression is up-regulated. MYB transcription factor specificity of MYB cis-elements combined. Select the SV40 enhancer in the period of MYB cis element TAACTG of bait to construct yeast plasmid using the yeast one-hybrid method, MYB element OsMYB2 identify binding specificity. The results show that, OsMYB2 MYB element specific binding, and the combined result of the downstream reporter gene expression is reduced, suggesting that this gene is a transcription inhibitor, the negative regulatory role in gene expression from.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
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