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Of the genetic basis of research has been the focus of the corn breeding work. The genetic characteristics of the the scientific identification corn germplasm quality of identification of germplasm, genetic resources evaluation, variety rights protection are important. The fundamental way to solve this problem is the identification, classification and finishing of germplasm material resources, on the basis of clear heterotic groups and heterotic patterns Germplasm and improved. In this study, SSR Markers system of Shaanxi Province corn germplasm research, build a of Shaanxi Province corn inbreds fingerprint database, and genetic diversity of Shaanxi Province, mainly maize inbred lines and hybrids. The main findings are as follows: 1, through the DNA extraction, PCR amplification, electrophoresis and silver staining optimization of key technologies integrated SSR analysis system from a uniform distribution in the 100 SSR primer pairs in 10 chromosomes screening 33 pairs amplified polymorphism with type, the better repeatability primer as Shaanxi Province corn germplasm fingerprint analysis of core primers. By SSR analysis, 23 maize inbred lines in Shaanxi Province were detected in 162 allelic variants of each primer pair 2-8 alleles detected an average of 4.91 alleles detected by each primer PIC distribution range of 0.406-0.884. Build 23 major maize inbred lines digitized fingerprints, calculate its probability of occurrence of 1.08 × 10-22, for the authenticity of the identification of inbred lines. SSR molecular marker Shaanxi Province, mainly maize inbred lines divided into four groups, namely improved Reid taxa the Siping head group, Lancaster the group and Ludahonggu group. Clustering results are basically consistent with known pedigree. Improved Reid microflora and of Siping head group containing 13 and six inbred lines, the proportion of heavier in the four groups. The two major groups of the genetic basis of abundant. 4, selected 29 pairs of core primers 26 Shaanxi Province corn hybrids SSR analysis detected a total of 148 allelic variation, the average number of alleles 5.1, range 2-9, mean polymorphism sexual amount of information 0.690. The genetic similarity between the varieties were ranged from 0.520-0.851, average 0.674, with a common parent varieties have a higher genetic similarity coefficient. 5, cluster analysis by UPGMA method can be divided into five major categories, 26 hybrids, heterosis mode for improved Reid × Siping head, Siping, head × foreign hybrids and synthetic varieties × foreign hybrids, their results and pedigree The analysis results are consistent. SSR technique screening core primers showed that the use of diversity of corn hybrids.
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