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Study on Expression of the Storage Protein and the Resistance to Powdery Mildew in Synthesized Wheat-rye Amphiploid

Author: ZengXiaoChuan
Tutor: RenZhengLong
School: Sichuan Agricultural University
Course: Crop Genetics and Breeding
Keywords: Wheat Rye Amphidiploid Epigenetic HWM-GS Gliadin GISH
CLC: S512.1
Type: Master's thesis
Year: 2008
Downloads: 29
Quote: 2
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Abstract

Polyploidization plays an important role in the formation process of the plant species. It is estimated that at least 50% or more than 70% of angiosperms have experienced one or more polyploidization events in the evolutionary process. The wheat Allohexaploid chromosomes into the nucleus for the closely related species tolerance is very strong, distant hybridization easier. Allopolyploid synthetic wheat and wheat related species is a material, not only can be used as breeding germplasm, varieties application on the production, at the same time to study the interaction of genomic, genome evolution, epigenetic material. Storage protein is important component of wheat grain, is closely related to the quality and wheat. Previous wheat grain storage protein gene locus and expression made a more in-depth studies. Wheat rye is also an important source of resistance to powdery mildew, the use of the building amphidiploid introduced rye resistance gene is an important means of breeding. This study by GISH, SDS-PAGE and A-PAGE identified two sets of synthetic wheat (MY11 Chinese Spring) and rye (AR106BONE) heterologous amphidiploid (CA, MA), and its grain analysis of storage protein expression and resistance to powdery mildew. The results are as follows: 1. GISH identification of two sets of 20 Grain apical cell in meiotic metaphase chromosome number are 2n = 56, 14 rye hybridization signal. SDS-PAGE and A-PAGE identification also prove that the allopolyploid and previous studies triticale allopolyploid many similar traits: the expression of most of the bands in the parent and pour maternal inheritance. The successful synthetic wheat - rye amphidiploid heterologous. 2. Several materials in the distant hybridization and chromosome doubling genetic variation in the absence of both the parent protein bands in the offspring different from the parent of this section with a new expression of the protein bands in the offspring. Mobility of rye slow bar with two sets of materials are not expressed, CA material in the Chinese Spring subunit 2 does not appear, at least two new subunits 7 up and down in the subunit expression. The MA material the female parent MY11 strip all expression. The description of the two sets of different wheat background material in a heterologous same rye amphidiploid not the same. 3.HMW-GS and gliadin in offspring genetic stability. HMW-GS composition analysis of the 20 materials found all the materials performance in gliadin composition of future generations becomes very rich. Means that the genetic stability of HMW-GS may be greater than gliadin. Gliadin polymorphism CA is less than in the two sets of materials MA, may mean that the Chinese Spring accepted exogenous chromosome more the distant hybridization synthetic allopolyploid easier. Rye AR multiple generations of selfing found the gliadin deletion rye alkali protein bands. To it as the descendants of the parent, Sec-1 expression in all partial expression and did not express several types. This may indicate that the abnormality has occurred changes: Sec-1 locus on chromosome rye AR 1R, attention should be paid to the heterologous polyploid Sec-1 expression regulation principle. Through field naturally induced Powdery Mildew found two sets of amphidiploid expression of disease-resistant genes from rye, showed the high resistance to powdery mildew.

Study on Expression of the Storage Protein and the Resistance to Powdery Mildew in Synthesized Wheat-rye Amphiploid

Abstract

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