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Study the Effects of PEG-PEI Nanoparticles Magnetic Ferrofluid-TK Targeting Therapy for Hu-man Primary Liver Cancer in Nude Mice Implanted with SMMC-7721

Author: LiuZuo
Tutor: PengJian
School: Central South University
Course: Surgery
Keywords: Primary liver cancer Nanoparticles Magnetic Fluid Thymidinekinase gene PEG-PEI/Fe3O4Nanoparticles Magnetic Fluid-TK GCV
CLC: R735.7
Type: Master's thesis
Year: 2008
Downloads: 230
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Abstract


Objective:To study the effects of PEG-PEI/Fe3O4 Nanoparticles Magnetic Fluid-TK targeting therapy for hu-man primary liver cancer in nude mice implanted with SMMC-7721.Methods:Human liver cancer cell line SMMC-7721 was implanted subcutaneously in nude mice to establish experiment model.Animals bearing liver cancer were randomly divided into five groups:control group,GCV（ganciclovir）group,Thymidinekinase group; PEG-PEI/Fe3O4 Nanoparticle Magnetic Fluid-TK.Different plasmids were directly injected into tumors and GCV was intrap-eritoneally administrated simultaneously according to different groups.The growth of tumors was observed and the pathology was examined as well.Serum AFP level was measured by radioimmunology,the ultrastructural change of tumor cells was studied by using electron microscopy,the expressions of MVD and VEGF were respectively detected with immunohistochemistry and the cell apoptosis in situ was detected by TUNEL.Results:The success rate to establish subcutaneous implanted liver cancer model in nude mice was 100%.The tumor volume,serum AFP level,VEGF and MVD expressions of PEG-PEI/Fe3O4 Nanoparticles Magnetic Fluid-TK group were lower than those in control group, Thymidinekinase group and GCV group（P＜0.05）and more apoptosis cells could be observed.The apoptosis index of Nanoparticles Magnetic Ferrofluid-TK group was higher than that of the latter（P＜0.05）.RT-PCR measured that shows the tumor mouse heart,the lung,the kidney has not seen the Thymidinekinase gene expression,but the tumour cell presents the Thymidinekinase gene expression.Conclusion:PEG-PEI/Fe3O4Nanoparticles Magnetic Fluid-TK inhibits the growth of tumor remarkably and becomes apromising new biological agent to treat human primary liver cancer.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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