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Objective: To investigate rapamycin (RPM) on renal ischemia-reperfusion injury in the efficacy and mechanisms. Reveals RPM on renal ischemia-reperfusion injury mechanisms of action and to seek the treatment of renal ischemia-reperfusion injury in an effective way. Methods: The first part: from the level of biochemical assays and cell morphology was observed RPM on renal ischemia-reperfusion renal function and ultrastructure. 30 male Wistar rats were randomly divided into three groups: sham operated (sham) group (resection of the right kidney, separation left renal artery, without blocking blood flow to the saline gavage), surgery (IR) group (resection right kidney, separation renal artery, blocking blood flow to normal saline administered before ischemia), drugs (RPM IR) group (resection of the right kidney, separation left renal artery, blocking blood flow before ischemia irrigation gastric administration of rapamycin (4mg / (Kg.d) × 3d, the last 2 h before surgery gavage) rat model of acute ischemic renal injury in the right kidney was removed, separating the left renal artery, blocking blood Stream 45 min, reperfusion 24 h, renal function and renal tissue was observed ultrastructural changes in the second part: the level of gene expression was observed RPM on renal ischemia-reperfusion rat renal apoptosis gene protein expression. 81 Wistar rats were randomly divided into three groups: sham operation group (resection of the right kidney, separation left renal artery, without blocking blood flow to normal saline administered before ischemia) in the surgery group (resection of the right kidney, separating the left renal artery, blocking blood flow to normal saline administered before ischemia), drug group (resection of the right kidney, separation left renal artery, blocking blood flow before ischemia intragastric administration of rapamycin (4mg / (Kg.d) × 3d, 2h preoperative surgical day gavage), postoperative administration to each observation date), ischemic group was divided by ischemia and reperfusion after reperfusion 0 h, 24 h , 48 h, 72 h, 4 observation phase, each observation group phase 9. analyzed by HE staining renal pathological injury, TUNEL assay renal cell apoptosis rate was detected by immunohistochemical SABC Fas, bcl -2 protein expression changes. Results: The first part: creatinine renal ischemia-reperfusion group (168 ± 37) μmol / L, blood urea nitrogen (22 ± 6) mmol / L, serum creatinine rapamycin group (113 ± 17) μmol / L, blood urea nitrogen (13.8 ± 2.3) mmol / L; 2 group, the difference was significant (P lt; 0.05). electron microscopy: operation group is a large necrotic tubular lumen cell debris clogging epithelial accumulation of lipid droplets in the cytoplasm, cell apoptosis signs; drug group slight tubular damage ultrastructure Part II: surgical group renal cell apoptosis was significantly higher (P lt; 0.05); medication group than Apoptosis surgery group was significantly lower (P lt; 0.05). Fas protein expression was negative sham surgery group 0 h reperfusion has little expression, 24, 48 and 72 h reperfusion were positive in 48 h reached a peak (P lt; 0. 05); mainly expressed in cortical tubules; drugs renal tissue Fas protein levels were significantly lower than the surgery group (P lt; 0.05). bcl-2 protein in the sham group showed a negative expression, 0 h of reperfusion surgery has little expression, 24, 48 and 72 h reperfusion were positive, 48 h and reached the peak (P lt; 0. 05); cortical tubule-based; drug group bcl-2 protein in kidney tissue surgery group was significantly higher than (P lt; 0.05). HE staining surgery group with varying degrees of renal tubular epithelial cell foci of necrosis and inflammatory cell infiltration, the drug significantly reduced renal tissue injury. Conclusions: The first part: RPM on renal ischemia-reperfusion injury in renal function and renal tissue protective effects ultrastructure second part: RPM on renal ischemia-reperfusion injury induced renal damage has a significant protective effect, the role of RPM may lower Fas, bcl-2 protein expression increased inhibition of apoptosis. Summary: RPM on renal ischemia-reperfusion in rats has a clear protective effect, is to explore the clinical prevention and mitigation of renal ischemia-reperfusion injury in an effective way world.
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