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Expression of Heparanase in Human Bladder Carcinoma T24 Cells and Its Effect on Cell Proliferation

Author: ShiPing
Tutor: YangMin；XuZhongHua
School: Shandong University
Course: Nursing
Keywords: RNA interference Heparanase Bladder cancer T24 cells Cell proliferation
CLC: R737.14
Type: Master's thesis
Year: 2008
Downloads: 50
Quote: 0
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Abstract


Objective 1. Research heparanase gene expression in human bladder transitional cell carcinoma T24 cells . Study -specific siRNA inhibition of heparanase gene expression in T24 cells . Study -specific siRNA on proliferation of T24 cells affect the method of in vitro chemical synthesis heparanase -specific double-stranded RNA (dsRNA), liposomes 2000 transfected T24 bladder transitional cell carcinoma , tetrazolium salt (MTT) assay cells growth of the reverse transcription polymerase chain (RT-PCR) to detect the heparanase gene mRNA expression detected by Western - Blot the heparanase gene protein expression . Results heparanase gene in human bladder transitional cell carcinoma cell line T24 was highly expressed in state . RNA interference silencing T24 bladder transitional cell cancer heparanase gene , the decline in the growth and vitality of the T24 cells , three different the interference cell viability decreased ( 28.55 ± 3.66) % , ( 27.20 ± 3.25 ) % and (37.29 ± 4.82)%, heparanase mRNA and protein expression levels were significantly decreased . Conclusion 1. Heparanase high expression the mPdqA and protein levels in human bladder transitional cell carcinoma T24 cells . Specific siRNA significantly inhibited 3 heparanase gene expression in T24 cells . Specific siRNA can effectively inhibit the proliferation of T24 cells , three different the interference cell viability decreased ( 28.55 ± 3.66) % , ( 27.20 ± 3.25 ) % and (37.29 ± 4.82 )%.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Bladder tumor
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