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Experimental Research of PLGA Tube on Repairing Sciatic Nerve Defect in Rats

Author: LiuXiaoFu
Tutor: ZengZhiCheng
School: Central South University
Course: Human Anatomy and Embryology
Keywords: PLGA sciatic nerve defect autograft spinal cord dorsal root ganglion (DRG)
CLC: R651.3
Type: Master's thesis
Year: 2008
Downloads: 65
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Abstract


Objective: To study the effects of PLGA tube on repairment of the sciatic nerve defect in rats.Methods: The PLGA tube were composed of 75% polylactic acid and 25% polyglycolic acid.36 adult Sprague-Dawley rats were randomly divided into the sham-operated group and the experimental group.The sham-operated group were not operated. The rats in experimental group were cut 10 mm nervous tissue from the right sciatic nerve and then randomly received PLGA tube bridging or autograft. The sciatic nerve function index (SFI) was observed at 4th, 8th and 12th week after transplantation. 12 weeks after transplantation, the label neurons in spinal cord and dorsal root ganglia (DRG) were assessed by Fast Blue retrograde tracing, the number, diameter and myelin thickness of the regenerated axons were measured by semithin and ultrathin section, and the triceps surae were weighted at last. All data were analyzed with the spss13.0 software and showed by (x±s), the comparison among multitude simple mean by One-Way analysis of variance (One-Way ANOVA).Results Muscles atrophy were detected at 12th week but not with anabrosis in each experimental group. The sciatic nerve index in the antotransplantation group were higher than PLGA group at 8th and 12th week(P<0.05).There was statistical difference between the experimental group and control group at 4th ,8th ,and 12th week(P<0.01).The number of labelled cells by the Fast Blue in PLGA group were significantly less than that in autograft group(P < 0.05).But there was no significant difference between control group and autograft group(P>0.05). The number, diameter and myelin thickness of the regenerated axons and the wet weight of triceps surae were significantly less than that in autograft group(P<0.05),and the difference between control group and autograft group was no statistic significance (P>0.05). Conclusion: The effect of PLGA tube bridging is worse than autograft bridging and control group, but it can recover the appearance and function of the sciatic nerve to some extent. So it is necessary to further study.

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CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Peripheral nerve
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