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Objective : To study the pulmonary perfusion with hypothermic cardiopulmonary bypass during lung preservation solution on lung tissue apoptosis . Methods: 20 cases of mitral valve replacement were randomly divided into lung protection group (n = 10) and control group (n = 10). Lung protection group pulmonary artery perfusion with hypothermic cardiopulmonary bypass during lung protective solution , the control group after pulmonary artery perfusion during cardiopulmonary bypass equal temperature saline solution. Patients were monitored cardiopulmonary bypass (CPB) after 0h, 6h, 12h, 24h respiratory function , and in start and stop the pump before CPB patients were taken after the right lower lung tissue (1.0 cm × 1.0cm × 1.0cm size ) biopsy specimens , DNA in situ end labeling immunohistochemical staining (TUN EL) detect apoptosis in lung tissue , and calculate the apoptosis rate ( ie apoptotic index ) , light microscope apoptotic morphological changes . Results: The group of patients after cardiopulmonary bypass lung protection 0h, 6h, 12h, 24h oxygenation index was higher ( respectively, t = 6.880, P lt; 0.01; t = 3.757, P lt; 0.01; t = 3.682, P lt ; 0.01; t = 2.416, P lt; 0.05); groups of patients after cardiopulmonary bypass lung number of apoptotic cells was significantly increased compared with before CPB (P lt; 0.01); groups of patients after cardiopulmonary bypass apoptosis in lung tissue Yield: lung tissue of lung protection group apoptosis rate (10.46 ± 1.96)%, while the control group lung tissue apoptosis rate (18.68 ± 1.34)%, the difference was statistically significant (P lt; 0.01) . Conclusions: ( 1 ) conventional cardiopulmonary bypass surgery can induce apoptosis in lung tissue ; ( 2 ) pulmonary artery perfusion with hypothermic cardiopulmonary bypass during lung protective solution can significantly inhibit apoptosis in lung tissue , reduce lung injury during cardiopulmonary bypass , suggesting that with lung protective effect.
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