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Objective: enzyme immunoassay (Enzyme Immune Assay EIA) and electrochemiluminescence immunoassay (ElectroChemiLuminescenceImmunoAssay, ECLIA) technology to detect HBV markers , explore this two detection of hepatitis B surface antigen ( HBsAg ) , hepatitis B e antigen (HBeAg ) and hepatitis B e antibody (Anti-HBe) indicators , sensitivity , specificity , reproducibility , at the same time explore two methods on the methodology and the practical application of the comparison , and ECLIA clinical blocking hepatitis B vertical transmission . Methods : by ECLIA determination and EIA serum and breast milk, the hepatitis B surface antigen ( HBsAg ) , hepatitis B e antigen (HBeAg) and hepatitis B e antibody (Anti-HBe) and HBV markers . Results: 1 . Applications ECLIA method to detect HBsAg precision , most large variation within ≤ 8.30% ≤ 15.33% , the biggest day variations , the sensitivity of HBsAg 0.05ng/mL, HBeAg sensitivity 0.03 NCU / ml . The HBsAg detection range 0.05-6250ng/ml. The EIA assay HBsAg precision ≤ 8.23% of variation ≤ 16.69% , the biggest day variations of HBsAg sensitivity 1.0ng/mL, HBsAg detection linear range 6.1-390.0ng/ml of . Newborn the 2.HBsAg positive mothers in childbirth in HBeAg -positive male : female 158:100 boy HBeAg positive rate was 50.60% , girl HBeAg positive rate was 49.24% . No statistically significant difference between both HBeAg -positive rate . Six months old , girls and boys, the infection rate fell to 0.60% and 1.05% respectively after . Conclusion The the : ECLIA detection of HBsAg high sensitivity , good reproducibility , wide detection range . Detection the HBeAg sensitivity may be higher. And ECLIA able to quantitative detection of hepatitis B markers , not only to avoid the window period of HBV markers low concentrations by ELISA missed the lack of , but also enables the early detection of the disease and monitoring of the disease at any time become possible , and effective guidance resistance off the vertical transmission of hepatitis B , the PMTCT succeed .
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