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HSP25,one of the small heat-shock proteins(smHSP),plays important roles in cytoskeleton protection,anti-injury and anti-apoptosis in cells.Recently,research showed that HSP can release from cells.It was reported that the plasma levels of Hsp27 were significantly increased in patients with ACS compared with the healthy people.It suggested HSP27 may be a new myocardial damage marker.This study was attempted to explore dynamic changes of serum HSP25(homologous to HSP27)levels during myocardial isChemia-reperfusion in rat and its clinical significance.In this experiment,the rat myocardial ischemia-reperfusion models were established by ligating the left anterior descending artery,leading to myocardial ischemia for 30min followed by reperfusion forlh,2h,4h,6h,12h,24h.Then blood was gotten from heart to observe dynamic changes of serum HSP25,LDH,CK,CK-MB,Mb and cTnI levels during myocardial ischemia-reperfusion in rat at different intervals.Then H9C2 cell line was treated by 40℃for 1h,40℃for 6h,43℃for 30minutes to detect release rate of LDH and concentration of HSP25 in cell supernatant.The results showed that:(1)Serum HSP25 levels significantly increased at 1h,reached peak at 2h and fellback gradually at 4h,6h,12h and 24h in I/R group rat,but still higher than sham group。(2) There was a positive correlation between serum HSP25 levels and serum LDH,CK,CK-MB,Mb levels in I/R group rat.(3)Both concentration of HSP25 in cell supernatant and release rate of LDH increased after H9C2 cell line was treated by 40℃for 6h,43℃for 30minutes compared with control group.Our results suggested that:(1) Small heat shock pritein 25 could release from damaged myocardium during myocardial ischemia-reperfusion injury and resulted in serum HSP25 levels increased.(2)In view of serum HSP25 levels obviously increased during myocardial ischemia-reperfusion injury and HSP25 possessed high sensitivity,serum HSP25 might be used as a diagnostic index at early stage of myocardial injury.
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