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Endophytic fungi living within a certain period of time in its life cycle within the plant tissue , plant tissue did not cause obvious disease fungi , recent studies indicate that endophytic fungi can produce and host the same or similar metabolism product. This feature enables the the endophytic fungi become resources to look for a new novel structure , active transfer compounds . Thus, this project chose Pinellia rhizoma pinelliae of representative materials to carry out the study of the biological activity of endophytic fungal metabolites , hoping to find a new active substance . This topic focus on internal endophytic fungi fermentation products pharmacological activity analysis application paper plates , DPPH method , ELISA method , MTT method , respectively, of its anti-bacterial , anti-oxidation , anti-viral and anti-tumor in vitro study of the pharmacological activity . From Pinellia of stems , total isolated 50 endophytic fungi on the above separation endogenous fungal fermentation culture , the pharmacologically active metabolite analysis . The experiment found that metabolites of endophytic fungi Pinellia have a good effect on the antioxidant , BX1401, BX1601, BX1501 The IC50 of the three strains were 1.8mg/mL , 2mg/mL , 5.9mg/mL . At the same time , it is found that strains BX0302, BX1401, BX1601, BX1701 on Gram-positive Staphylococcus aureus have different levels of antibacterial activity of strains BX1001 , BX1601 , BX1602 , BX1501 , BX2501 , BX1902 Gram-negative Pseudomonas aeruginosa have a significant effect . However, the metabolites Candida fungus white good antibacterial activity . On the experimental results , Pinellia endogenous fungal metabolites no significant anti- hepatitis B virus activity . Metabolites of Skov3 cells have different levels of cytotoxic activity . By experiment again confirms the endophytic fungi is a good source of drugs . At the same time , Pinellia the endogenous fungal metabolite good antioxidant activity and Pinellia overlap with their antioxidant activity . Endophytic fungi good stress tolerance and diversity , the material foundation for the development of Chinese medicine again .
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