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Objective: To observe the Shenqi Fuzheng injection of doxorubicin (Adriamycin, ADR)-induced heart failure in rat ventricular remodeling, explore its mechanism to provide a scientific basis for the clinical use. Methods: cardiotoxicity of doxorubicin-induced rat model of heart failure. 60 male SD rats were randomly divided into six groups: normal group, the doxorubicin model group, captopril group Shenqifuzheng injection solution (hereinafter referred to as Shenqi) low-dose group, Shenqi Fuzheng injection in dose group, Shenqi righting injection high-dose group. The spirit of the rats in the experimental process, activities, eating, after eight weeks of treatment, determination of left ventricular hypertrophy index, HE staining observed myocardial myocardial pathological changes of immunohistochemical observation myocardial Ⅰ, Ⅲ collagen expression, RT- PCR method of myocardial MMP-3, the semi-quantitative analysis of TIMP-1 mRNA. Results: 1, Determination of cardiac function: cardiac function model group (± dp / dtmax) was significantly lower than that of the normal group (P lt; 0.01); compared with the model group, the medication group can improve heart function to varying degrees: the Shenqi middle dose group significant effect (P lt; 0.01), the Shenqi large dose group no significant sex change (P gt; 0.05). 2, cardiac hypertrophy index: heart failure models of heart body and left ventricular hypertrophy index were significantly higher than the normal group (P lt; 0.01); compared with the model group, drug treatment group can improve myocardial hypertrophy situation: Among them, the Shenqi in dose group efficacy was significantly (P lt; 0.01), the Shenqi large dose group showed a significant change (P gt; 0.05). 3, Ⅰ, Ⅲ collagen expression: immunohistochemical staining results showed that the model group Ⅰ, Ⅲ collagen expression was significantly higher than the normal group (P lt; 0.01); compared with the model group, drug treatment group can be varying degrees reduce the expression of collagen, which the Shenqi in the dose group Ⅰ, Ⅲ collagen expression was significantly decreased (P lt; 0.01), the Shenqi large dose group was no significant difference (P gt; 0.05). 4, RT-PCR results show that: Compared with normal group, the the model group MMP-3mRNA significantly higher (P lt; 0.01), TIMP-1mRNA significantly lower (P lt; 0.01); the Shenqi small doses in dose and Cato the Plymouth group the expression of MMP-3mRNA significantly lower than that in the model group (P lt; 0.01, P lt; 0.05); TIMP-1mRNA significantly higher than that in the model group (P lt; 0.01, P lt; 0.05); the Shenqi big dose of MMP -3mRNA, TIMP-1mRNA expression with model group, no significant difference (P gt; 0.05). Conclusion: 1, Shenqi Fuzheng injection can improve heart function. 2 Shenqifuzheng injection liquid can reduce heart failure left ventricular hypertrophy situation. Shenqi Fuzheng injection can improve myocardial I., Ⅲ collagen expression. Shenqifuzheng injection fluid can reduce MMP-3 expression or inhibit its activity, raised TIMP-1 expression.
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