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Rhizoma Alpiniae officinarum is drying plant rhizomes of Alpinia officinarum Hance. Mainly contains: Essential oils, flavonoids, diphenylheptane category, phenylpropanoid category, glycosides and trace constituents. With the effect of warming the middle warmer, cold-dispelling, promoting digestion, and alleviating pain, it commonly used for the treatment of abdominal psychroalgia, gastrofrigid vomiting,belching, etc. In recent years, both at home and abroad, studies of Galangal were mainly centered on flavonoids. In this experiment diphenylheptane constituents are studied, and a new modern medicine,“DPH Gel”for the treatment of pain is studied and prepared.In this experiment, with diphenyl heptane A as indicator, Galangal medicines were screened.Effective fraction in Chinese medicinal materials was extracted by supercritical fluid extraction technology and SFE extraction was obtained. Molecular distillation technology was used for further purification of SFE extraction from Rhizoma Alpiniae officinarum. By the method of single-factor experiment, purification process was optimized: temperature 90℃, the vacuum 5 Pa, super critical extraction pre-heated in 60℃, scraping membrane rotor speed 260 to 280 r·min-1, flow rate 2 mL·min-1.Effective fraction in extracts by molecular distillation technology （shorted as“DPH extracts”）were as identified through LC-MS. Quality control methods for DPH extracts were studied.Identified through animal experiments, the LD50 value of SFE extracts and DPH extracts were as follows: 1.3377 g / kg and 10.641 g / kg. And through acetic acid writhing test, the analgesic efficacy of Galangal extract was confirmed. A linear dose-effect relationship of Galangal extract was identified, which is ED50 464.39 mg·kg-1. Based on the physical and chemical properties of raw materials and the characteristics of gel, DPH gel formulations process was optimized. Optimum: Carbomer was uniformly sprinkled on the surface of distilled water, and standing to the full swelling, then 3% Carbomer hydrogel matrix is prepared. Galangal extract was dissolved into ethanol,then Azone,glycerin,propyleneglycol, and ethyl Nipagin were joined and blended into uniform solution. The solution was added in the matrix, stirring, then triethanolamine was slowly added. Stirring, water was added to prepare DPH Gel.Quality control methods for preparations were studied by ultraviolet spectrophotometry. Contents of main active ingredient in the preparation were determinated. Standards for quality control of the preparation were established.
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