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Study on Formation Process and Cultivating Conditions of Mycelial Pellets

Author: ZhuHong
Tutor: MaFang
School: Harbin Institute of Technology
Course: Microbiology
Keywords: Aspergillus Mycelial pellets Of aniline degradation of bacteria Immobilized
CLC: X703
Type: Master's thesis
Year: 2007
Downloads: 322
Quote: 1
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Abstract


In the wastewater treatment process, the direct dosing bacterial Although it is simple, but the effects of dosing bacterial easy loss, or other microorganisms engulfed. Immobilized microbial technology may be free of microorganisms fixed on a carrier, to make it highly dense and remain active, the wastewater treatment system is efficient, stable operation. The mycelium ball is a microbial fermentation process naturally formed particles, it is a good biological activity, the settlement rate, easy-to-solid-liquid separation, and for the treatment of heavy metal wastewater and dyeing wastewater. However, the waste water is a very complex hybrid system, complex water quality ingredients, and different environmental factors on the mutagenic effects of microbial and other factors is difficult to form the purebred single mycelial pellets. Therefore, a single mycelial pellets as a carrier, the use of mixed cultures since immobilization technology, the establishment of mixed flora consisting of micro-ecological environment, the immobilized microorganisms play together, will become the focus of research in the field of wastewater treatment. Determined on the basis of mycelial pellets optimal medium single the mycelium ball formation process and its impact factors, as well as the formation of mixed mycelial pellets and influencing factors, at the same time, the purification efficiency of mixed mycelial pellets A preliminary study. Form mycelial pellets optimization niger Y3 medium, were selected sucrose, ammonium chloride for optimum carbon and nitrogen sources the the Mg 2 and Fe 2 mycelium ball the growth of important trace elements. Proven a single the mycelial pellets into a ball conditions and formation process. Inoculated with a spore concentration of 104 / mL, the initial pH of 4 to 6, at 37 ° C, 160rpm shaker 4D, the formation of the A. niger Y3 mycelial pellets of various aspects of performance. Single the mycelium ball formation process can be summarized into three stages: the spore nuclei germination grow mycelium; growth of mycelium extending the role of external forces to form a mycelium ball; mycelium continues to grow, culture conditions deteriorate, mycelial pellets autolysis. To study the formation of mixed mycelial pellets, experiments show two ways to self-flocculation and adsorption can cultivate hybrid mycelium ball. Aspergillus spores and bacteria co-cultured to form a mixed mycelial pellets through the following four stages: the bacteria involved in spore cohesion, and the spores together form saturated nuclei; saturated nuclei bacterial growth and reproduction, spore germination grow mycelium; shear in the flow extending growth under the action of shear forces, mycelium side edges around the nuclei wound bacteria wrapped therein and make it adhere in the hyphae; mixing mycelium ball formation, and further growth. Experimental adsorption to form a mixed mycelial pellets, aniline degrading bacteria bacterial suspension initial pH 6.0 to 7.0, shaking speed of 140 rpm at 25 ~ 35 ℃, the ball diameter of 1.2 ~ 1.8mm single mycelium ball of aniline degrading bacteria Immobilized effect. Mixed mycelium of Aspergillus Y3 aniline degrading bacteria have a certain degree of purification performance ball the aniline removal rate as high as 82.45%. The scanning electron micrographs show to The immobilized microbial adhesion firmly in hyphae grow well, a living substance carrier technology development has laid a theoretical foundation.

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CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > General issues > Wastewater treatment and utilization
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