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1, using of Karber (Korbor) studied Cu 2 and Cd 2 of the Bellamya (Bellamyapurificata) 96h acute toxicity, the results showed that: Cu 2 , Cd 2 the LC the B.purificata 96H 50 and 95% sent a letter interval were 0.382 (0.314-0.464) mg / L, 3.390 (2.819-4.076) mg / L. 2, using the method of exposure to heavy metals of different concentrations of copper sulfate (Cu 2 0 mg / L, 0.005 mg / L and 0.01 mg / L, 0.02 mg / L, 0.05 mg / L on Bellamya liver and gill tissue content of mitochondrial ROS) in different exposure time 0-10d under 0-14d the pear ring Bellamya CAT, SOD, GST of activity, GSH and MT content, as well as DNA single strand breaks extent, the results showed that: Cu 2 the Bellamya liver and gill ros, CAT, SOD, GST, GSH, MT and DNA fragmentation were significantly affected, showing the time and dose effects . ROS levels with the exposure time and dose showed a rising trend, show a clear time-dose effects. The former 4 d, SOD and CAT previous 3 d of enzyme activity generally exhibit induced trend, GST induction of enzyme activity in the first 4 d, with the exposure time, decreased activity, showing inhibition trends; over time further growth to 14 d, 0.005 mg / L dose groups enzyme activity is maintained at the normal fluctuations in the vicinity of 0.05 mg / L dose group of the enzyme activity is inhibited, 0.01 mg / L dose groups enzyme activity was induced by 0.02 mg / L dose group activity in the liver showed induction in the gills were suppressed. Similar changes in liver and gill GSH content and GST, the to demonstrate induction effect in a short period of time, the liver GSH 5d prior to the exposure in the induced state, gill GSH 4d are exposed in the induced state, with the exposure time , 0.005 mg / L dose group showed induction, 0.05 mg / L dose group was suppressed. MT during the whole experiment are in the induced state. Liver and gill tissue DNA single strand breaks with Cu 2 exposure dose and exposure time significantly. 1d after exposure to Cu 2 , F value is rapidly reduced, and then, F value is increased, the repair of DNA showed a certain degree of injury prompt, F 2d reach a higher point value ; followed by the degree of DNA single strand breaks continues to grow, the F value showed a continued downward trend. Liver tissue in the first 10-when 14 days 0.005,0.01,0.02 and 0.05 dose groups F value is not significantly different. 3, using the method of exposure to heavy metals, with different concentrations of cadmium chloride (Cd 2 0 mg / L and 0.05 mg / L and 0.10 mg / L and 0.20 mg / L, 0.50 mg / L) under different exposure time (0-14d) pear-shaped ring Bellamya hydrogen peroxide enzyme CAT, SOD, GST of activity, GSH and MDA content. The results showed that: the Cd 2 Bellamya liver and gill CAT, SOD, GST, GSH and MDA were significantly affected, showing time and dose effect. Liver SOD activity in the first 10 d induced state; 0.05, 0.10 and 0.20 dose group in the gill tissue SOD activity has been in the induced state. 0.05 and 0.10-dose group liver CAT is induced in the exposed ago 7d activity, then the activity decreased; dose group of 0.20 and 0.50 in the first day when the enzyme activity was significantly increased, followed by enzyme activity has been decreased until the 10d activity very significant is inhibition; gill organizations in each dose group CAT exposed 7d in the induced state (0.50-dose group, except 4d), and then the enzyme activity declined sharply in the first 10-14d enzyme activity was significantly inhibited. 0.05 and 0.10 dose group liver GST activity has been in the induced state; 0.20 dose group first 7d is significantly or very significantly induced state; 0.50 dose group in the first 1d has been very significantly induce and then decreased. GST trends in the gill tissue of the liver are similar. Each dose group in the induction of the first 5d state, from the first 7 days after the rapid decline in enzyme activity, enzyme activity was inhibited in the first 10-when 14 days. Liver and gills in each dose group GSH first 7d has been in a significant or very significant induction of state (0.50 dose group liver 1d and gills except 4d), the enzyme activity in the first 10 days when there is a decline in the process, and then enzyme activity picked up, to 14d are higher than that of the control group. MDA content in the liver tissue exposed 1d decline, followed by the MDA content increased, until 4d rose to the highest value. MDA content then began to fall. The gill tissue dose group of 0.20 and 0.50 in MDA content in 2d peak, 0.05 and 0.10 dose group decreased after the first 4d peak fluctuations in the middle to 14d with the control group, no significant difference. Liver and gill tissues increase in DNA single-strand breaks (expressed as a reduction of the F value). Liver exposed before 4d, F value decreased gill tissue exposed to the first 1d DNA damage significantly significantly or very significantly, the subsequent increase in the F value, a certain degree of repair Show fracture DNA, and DNA single-strand breaks extent to continue emphasis, F value continued to decline, until the 14d.
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