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Researches on Rapid Detecting Methods of Antibiotic Residues in Raw Milk and Milk Adulteration

Author: ZuoBinBin
Tutor: YaoXinKui
School: Xinjiang Agricultural University
Course: Animal Nutrition and Feed Science
Keywords: Raw milk Adulteration Detect Kit
CLC: TS252.7
Type: Master's thesis
Year: 2010
Downloads: 213
Quote: 0
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Abstract


This thesis raw milk conventional adulteration detection methods , the method is designed to be quickly detected the lack of on-site laboratory conditions , require rapid detection methods , high accuracy , simple operation , low cost , easy to carry , so that the raw milk the acquisition of a convenient, fast and accurate. Test : in a dairy enterprise data collection , analysis , to arrive at the main raw milk adulterated substances , the amount added , and adulteration causes and antibiotic residues , for targeted detection method . The results show that : the raw milk adulteration, residual material ( salt, starch , alkali , nitrite , creamer , hydrolyzed protein , antibiotics ) . Adulteration due to adulteration : In order to achieve the acquisition criteria ; prevent raw milk deterioration and prolong shelf life ; increase revenue. Test 2 : Is the antibiotic assay kit for trial , with a detection kit ( the Daier Wo rapid assay and of Dai Erwo slow assay ) . The results show that : the two kinds of methods can be detected under the lower limit ; the Dai Erwo rapid detection assay time is short , the detection time for 10 minutes , but poor reproducibility , the procedure is cumbersome , operate, and require training to operate accurately , otherwise it will lead to reagent contamination ; the Dai Erwo slow assay repeatability , simple operation , no training can operate , reagents does not pollute , but detection need 2.5 - 3h . Test 3 : 12 adulteration of raw milk substance detection method ( Glauber's salt , salt , nitrite , formaldehyde , ammonium salts , edible base, starch , sulfides , dextrin, hydrolyzed protein , boric acid , borax ) , the order of reagent addition , the added amount , the result of the color change , the detection limit , part of the interference , the accuracy of the method , the sensitivity, accuracy , reagent shelf life . The results show that : the detection method has easy operation , good coloring effect , the phenomenon is obvious , low detection limit . Adulteration easy to judge and grasp the detection of low-cost , short detection time characteristics at room temperature and the reaction can be carried out smoothly , it should be made ??of the kit , and can be used outdoors and special places , easy to promote in the actual production application .

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CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > The dairy processing industry > Product standards and testing
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