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Goat FSHβ Gene and Its Correlation with litter size

Author: DuLin
Tutor: XuGangYi
School: Sichuan Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Goat FSHβ gene PCR-SSCP Polymorphism Reproductive performance
CLC: S827
Type: Master's thesis
Year: 2008
Downloads: 94
Quote: 2
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Abstract


FSH is a glycoprotein pituitary gonadotropins , affect animal reproductive system development and male animals sperm production , commonly used in common with superovulation and embryo transfer is to promote follicle growth, development , maturation and ovulation essential hormones . Sichuan Agricultural University, Tianfu sheep after 28 years of complex multi- species hybrids bred breeding meat goat breeding success of new lines . In this experiment, the sheep industry , Sichuan Agricultural University Animal Husbandry Co., science centers and Huiyuan Tianfu bred 166 sheep , 101 goats and 67 sheep for the study Chengdu Ma on FSHβ gene PCR-SSCP to study the genetic diversity and its relationship with the number of births correlation analysis . This study is based on the GeneBank cattle , sheep and pigs species FSHβ gene sequences , PCR primers were designed to amplify goat FSHβ gene 5 'regulatory region , exon 1 and exon 2 . The results showed that : FSHβ gene 5 'regulatory region , found a SNP site is A712G. FSHβ gene primers 1 in Tianfu sheep and Boer goats are moderate degree of polymorphism (0.25 0.05). There lambing records for mutation sites and goats parity with its litter size in least-squares analysis, the results showed that : AA genotype Tianfu least squares mean litter size of sheep firstborn BB genotype were more than 0.39 (P <0.05), second child , third child more than the first-born litter size were 0.6 (P <0.05), 0.87 P <0.01). AA genotype in Chengdu Ma goat litter size in the third child of up to 2.60 , AA genotype in three varieties of goat second child and third child ( including the above ) in high yield stronger than the first child effect (P <0.05); AA genotype in all varieties of each parity litter size than BB genotype (P <0.05. present study found a pair of primers and goat breeding traits related, but needs further research .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Goat
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