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Studies on Extracting Method of Momordica Saponions and Screening of Bitter Gourd(Momordica Charantia L.) Varieties with High Momordica Saponions Content

Author: QiuHong
Tutor: WeiYouYing
School: Shandong Agricultural University
Course: Olericulture
Keywords: Bitter gourd Saponins Determine Extract Variation Correlation
CLC: S642.5
Type: Master's thesis
Year: 2008
Downloads: 223
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Abstract

Starting with optimization bitter gourd saponin content determination method to determine the optimum extraction conditions of ultrasonic bitter gourd saponins, and then study the bitter gourd different growth stages and plant vegetative organs saponins content changes, and varieties of bitter melon saponins content conduct a study of the variability and bitter melon fruit traits with bitter melon saponins content, for the development to achieve the goal of effective cultivation of bitter gourd high saponin content, use and breeding of new technology to provide a theoretical basis. The results of this study show that: 1. Determine the the bitter melon saponins Determination method to create colorimetric determination of the content of bitter melon extract saponins, provide the basis and method for the quality control of the bitter gourd. Conditions optimized by contrasting colors, select the best conditions: vanillin - acetic acid - perchloric acid as chromogenic reagent, heating time 15min, the heating temperature of 60 ° C, the chromogenic reagent dosage of 0.2 ml 5% vanillin in glacial acetic acid, 0.8 ml perchloric acid. The results show that the high precision of the measurement method, the color stability within 40-60min, recoveries, the average recovery rate was 101.22%, RSD was 1.064% (n = 5), and simple operation of the equipment do not ask for much, is a rapid determination of the bitter melon saponins good method. 2. Optimize the the bitter melon saponins ultrasound to extract the optimal conditions to study the optimum extraction conditions of ultrasonic extraction of bitter melon saponins. The the study extraction temperature, ethanol concentration, solid-liquid ratio and extraction time and other factors at different levels of bitter melon saponins extracted rate. By spectrophotometry, bitter gourd content of total saponins. The results showed that the optimum combination of ultrasonic extraction: 70% ethanol, solid-liquid ratio of 1:30, the oscillation frequency of 60%, at 70 ° C 30min / ultrasonic extraction, extraction was repeated twice. The method is simple, rapid, accurate, and can be as bitter gourd products extraction and quality assessment. 3. The bitter melon saponins content of different harvest application of plant chemical methods to study the bitter melon saponins content of Fruit. Using ultrasonic extraction and spectrophotometric determination of saponins content of the bitter gourd different varieties 1 # and 53 # different harvest period. The results show that, bitter melon fruit The saponins content with harvest time low - high - low trend, in which the highest total saponin content about 15d for medicinal bitter gourd best harvest period. The presence of saponins content of bitter gourd different harvest varieties significant interaction effect. Determination of total saponin content in different balsam pears should be determined in the same harvest period. 4 different vegetative organs bitter melon saponins content # 62 monoclonal bitter gourd roots, stems, leaves, seeds of bitter melon saponins content was measured to study the difference of total saponin content. The results showed that the content of the fruit of the bitter melon saponins in different organs, the highest for 1.644%. The seeds, leaves, stems turn reduced root the lowest content of 0.0744%. 5. Study the variation of the total saponin content in bitter gourd varieties saponins content was measured 15 bitter gourd varieties. The results showed that: the bitter gourd different varieties saponins content differences reached significant levels, and bitter gourd varieties saponin content genetic variation exists. The bitter melon saponins content between 0.90% -2.91% change, the highest saponin content varieties # 63. 6. Bitter melon fruit traits with bitter melon saponins content of the tests on 10 of the 15 balsam pears fruit traits research. The results show that bitter melon soap glucoside content and fruit color of genetic-related coefficient was extremely significant with a positive correlation; and bitterness, fruit-shaped genetic coefficient showed a significant positive correlation; fruit aneurysm, fruit length of genetic-related coefficient was significant with negative correlation. If tumor, fruit color, fruit and fruit thorn 4 appearance quality traits between closely related, often a trait changes will bring the other traits change. Therefore, by the choice of appearance quality, high saponin bitter gourd indirect selection.

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