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The MAS technology nurturing cold anti- plague rice varieties Kongyu 131 (Pi1/Pi2)

Author: YangFan
Tutor: LiRongTian
School: Heilongjiang University
Course: Biochemistry and Molecular Biology
Keywords: Rice Pi1 Pi2 SSR marker marked-assisted Selection (MAS)
CLC: S511
Type: Master's thesis
Year: 2008
Downloads: 61
Quote: 0
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Abstract


Rice blast was one of limited factors to rice production in cold region. The main reasons of Heilongjiang rice variety suscepctible to rice blast included that the one was the narrow resource in genetic breeding resistant to blast, the other was that classical breeding can not utilize genes resistant to blast in indica rice effectively, quickly and precisely. Pi1 and Pi2 were wide-spectrum and dominant genes resistant to blast in indica rice. In order to introduce Pi1 and Pi2 genes into the genome of Heilongjiang rice variety, developing the rice line Kongyu131( Pi1 / Pi2), the back crossing program was carried out by MAS with BL6 as a donor parent and Heilongjiang rice variety Kongyu131 as receptor parent. So, at first, the polymorphism of SSR molecular marker was screened between the parents.While, F1 of the receptor and donor parents were obtained and identified with SSR molecular marker. Then positive F1 population was used as female parent, backcrossing with receptor parent Kongyu131. BC1F1 population was identified in order to conduct foreground and background selection with SSR, and selected plants resistant to blast were studied with artificial inoculation. The main results were showed below.1. SSR markers RM144 and AP22 , which showed polymorphism between receptor parent and donor parent and closely-linked to Pi1 and Pi2 genes , were used in positive selection to develop Kongyu131(Pi1/Pi2) by MAS.2. 24 SSR markers were selected carried out background selection in developing Kongyu131(Pi1/Pi2).3. Sexual hybridization was conducted in donor parent and receptor parent , the hybrid were identified from F1 population by AP22 marker, and were used as female parents of backcrossing with Kongyu131 to obtain BC1F1 population.4. BC1F1 population plants were analyzed based on foreground selection and background selection, and obtained 9 plants with Pi1 and Pi2 genes, its background recovering to Kongyu131 genome with an average of 70% and being resistant to rice blast strains of Heilongjiang province.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
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