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This study to of Hubei Province copper Lushan Tongshankou mine acidic mine water as the material, conducted At.f bacteria isolated and purified, and its growth characteristics, leaching effect, the leaching mechanism of preliminary research. Acidophilic Thiobacillus ferrooxidans 9K liquid medium enrichment culture and purification of water samples purified strains by 16S rDNA identification, named C1. The C1 bacteria optimum pH of 2.0, and the growth temperature of 30 ° C, inoculum size 10% and shaker speed 150 r / min. At a temperature of 30 ° C, initial pH of 1.5, shaking speed 135 r / min, inoculum size of 2%, the ore particle size of 74-97 μm added FeSO4 · 7H2O 44.7 g / L pyrite 15 g / L, and phosphate rock conditions of 10 g / L, after 28 d of leaching, leaching the phosphorus rate is 77.19%. Select the initial pH, inoculum size, speed, ore particle size four factor orthogonal experiment, the impact of various factors on experimental results, a significant order for the pH gt; inoculum size gt; Speed ??gt; ore particle size. Tween-type surfactant was added in the leaching medium can be effectively improved dip phosphorus rate, when Tween 20,40,60,80 dosage of respectively 10 g/m3 and 10 g/m3 and 10 g/m3 and and 50 g/m3 effect Baptist phosphorus rate were increased by 31.00%, 36.48%, 45.19% and 41.91%. After 4 d leaching using ordinary leaching method, 9K, 9K S and Waksman medium, dip phosphorus were 57.07%, 76.75%, 58.13%, while the batch leaching method used in the three media After 22 d of leaching, the leaching of phosphorus rate were 48.41%, 58.89%, 25.16%, respectively. Ultraviolet radiation mutagenesis C1 bacteria in the in pyrite energy medium, 3 min, when using mutant strains of rock phosphate after 14 d of leaching, leaching the phosphorus rate is 20.07%, than the untreated original strains 31.69%; the microwave radiation mutagenesis C1 bacteria 10 s, this mutant strain after 14 d of rock phosphate leaching, leaching of phosphorus rate of 21.96 percent, 51.87 percent higher than the original strain, the leaching rate; concentration. 0.02 mol / L nitrite mutagenesis C1 bacteria, mutagenic bacteria leaching phosphorus rate than the initial bacteria increased 35.01%; C1 bacteria 15 min with 0.6% diethyl sulfate mutagenesis, mutagenic bacteria leaching phosphorus rate than the original strains increased 31.03%; 1.0% hydroxylamine hydrochloride of mutagenesis C1 bacteria, the the mutagenesis bacteria Baptist phosphorus rate higher than the original strain of 33.31%; After microwave and concentration of 0.02 mol / L nitrite the compound mutagenesis C1 bacteria, this lure the stain fungi Baptist phosphorus rate of 24.86%, 28.14% higher than the starting strain; to C1 bacteria were diethyl sulfate complex mutagenesis and hydroxylamine hydrochloride, phosphate rock leaching rate is lower than the blank. C1 bacteria in mineral surface to reach equilibrium after 40 min, the maximum adsorption capacity of 41.48%. C1 bacteria after 21 d of leaching of phosphate rock, dip phosphorus rate of 25.71%, an increase of 1.59 times the 9.89% of the chemical leaching. Added to the medium a certain amount of glycine, lysine, and glutamic acid can be improved rate dip phosphorus, and after a certain amount of leucine, dip phosphorus rate decreased. The optimum amount of glycine, lysine and glutamate were 0.5 g / L and 0.5 g / L, 1 g / L Baptist phosphorus rate of 24.07%, 12.03%, 7.6%, including lysine and no glycine obvious role of glutamate. Adding a certain amount of fructose in the medium can be increased rate dip phosphorus, and after a certain amount of arabinose, dip phosphorus rate decreased. Fructose optimum dosage is 0.5 g / L, then dip phosphorus ratio 15.54%.
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