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A phosphorus limit 8 microalgal chlorophyll fluorescence characteristics and growth at a temperature of 20 ± 1 ° C, salinity of 31, light intensity was 100μmol/m2 · s conditions, respectively containing different nitrogen concentration (0μM , 100μM, 880μM, 7040μM), different phosphorus concentration (0μM, 10μM, 36.3μM, 290.4μM) medium for microalgae Ocean University of China and other genebank saved creek embankment tower dinoflagellates strains (Tahitian Isochrysis gatbana), Zhanjiang other dinoflagellates (Isochrysis zhanjiangensis), P. viridis (Pavloca viridis), Chlorella (Chlorella sp.), oculata (Nannochloris oculata), of algae (Pyramimonas sp.), closterium ( Nitzschia closterium f. minutissima), Phaeodactylum (Phaeodactylum tricornutum) were cultured microalgae research 8 in a disposable training process, different nitrogen and phosphorus concentration on chlorophyll fluorescence parameters, chlorophyll content and cell density. Determination of the main parameters are: photosystem II (PSII) maximum photochemical efficiency (Fv / Fm), PSII actual photochemical efficiency (ΦPSII), photosynthetic electron transport rate (ETR), photochemical quenching (qP) and non-photochemical quenching (qN, NPQ). ANOVA results showed that different concentrations of nitrogen and phosphorus on 8 microalgae and growth of the chlorophyll fluorescence parameters were significantly affected (P lt; 0.05). Multiple comparisons showed that 8 microalgae photosynthesis and growth optimum nitrogen and phosphorus concentrations were 880μM, 36.3μM. 2 limiting nutrient after adding eight microalgal chlorophyll fluorescence parameters of the instantaneous change in (NIFT reaction) were used for nitrogen deficiency and deficiency medium Ocean University of China and other microalgae genebank saved creek embankment strains tower dinoflagellates , Zhanjiang dinoflagellates, P. viridis, Chlorella, Nannochloropsis, of algae, closterium, Phaeodactylum cultured, culture temperature was 20 ± 1 ° C, salinity of 31 , light intensity was 100μmol/m2 · s. Restrictions 3,5,7 d after adding the sample directly to the nitrogen and phosphorus, nitrogen and phosphorus study after adding eight kinds of algae chlorophyll fluorescence parameters of the instantaneous change (NIFT reaction) situation. Determination of chlorophyll fluorescence parameters F (instantaneous fluorescence), Fm (maximal fluorescence), PSII actual photochemical efficiency (ΦPSII), photosynthetic electron transport rate (ETR), photochemical quenching (qP) and non-photochemical quenching (qN , NPQ). The results show that, in this experiment 8 microalgae, add NO3 - N and NH4-N, in addition to other dinoflagellates tower Creek embankment strains and oculata, the other six microalgae overall fluorescence parameters the same trend, the instantaneous value of the fluorescence F declining, NPQ transient increases. Overall, with the added NO3 - N compared to NH4-N is added, 8 microalgae NIFT reaction more pronounced. In addition, the size of transient response trends and nutrient limitation related to the time; 8 Microalgae adding the phosphorus, in addition to outside qP instantaneous change in fluorescence parameters are the same tendency, F, Fm, ΦPSII, ETR instantly reduced, and qN, NPQ is instantly increased. 3 limiting nutrient salt is added after 8 microalgae within 24h of chlorophyll fluorescence recovery of nitrogen were treated with different concentrations (0μM, 100μM and 880μM) and different phosphorus concentration (0μM, 10μM and 36.3μM) medium for China Ocean University of microalgae genebank saved creek embankment tower dinoflagellates such strains, Zhanjiang dinoflagellates, P. viridis, Chlorella, Nannochloropsis, of algae, closterium, Phaeodactylum conduct culture, the culture temperature was 20 ± 1 ° C, salinity of 31, light intensity was 100μmol/m2 · s. 7d, respectively, was added to the culture of the limiting nutrients nitrogen, phosphorus, chlorophyll fluorescence parameters were measured within 24h recovery. The main parameters determined are: PSII maximum photochemical efficiency (Fv / Fm), PSII actual photochemical efficiency (ΦPSII), photosynthetic electron transport rate (ETR), photochemical quenching (qP) and non-photochemical quenching (qN, NPQ). The results show that, in the former 7d of the training process, 8 microalgae chlorophyll fluorescence parameters Fv / Fm, ΦPSII and ETR were significantly lower, and with lower nutrient concentrations, incubation time, dropping gradually increases ; adding nitrogen and phosphorus, the eight microalgae chlorophyll fluorescence parameters Fv / Fm, ΦPSII and ETR were up, including nitrogen deficiency, the largest increase in phosphorus groups, and qP, qN and NPQ value changes due to different algae species and different nutrient limitation and different.
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