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Effects of Da Cheng Qi Tang Animal Plasma on IP3R mRNA Expression in Interstitial Cells of Cajal

Author: ZhangDongLiang
Tutor: QiQingHui
School: Dalian Medical University
Course: Traditional Chinese Medicine
Keywords: Dachengqitang Pharmacokinetics Emodin Interstitial cells of Cajal Trisphosphate receptor
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 137
Quote: 1
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Abstract


Background: Chinese medicine pharmacokinetic study of the active ingredient of traditional Chinese medicine in the body's absorption, distribution, metabolism and excretion dynamic variation of an interdisciplinary. Dynamic variation of the traditional Chinese medicine active ingredient in vivo studies can clarify its mechanism of action, and interpretation of the traditional theory of Chinese medicine with modern science. Through mastery of the body absorption, distribution, metabolism and excretion characteristics can develop reasonable dosing regimen to promote clinical rational drug use, improve the level of clinical. Dachengqitang pass in the capture behalf of prescriptions, have a significant effect in the treatment of gastrointestinal motility disorders diseases. Emodin honokiol its main medicinal components involved in the regulation of the mobilization of intracellular calcium stores promote signal transduction, enhance gastrointestinal motility. Gastrointestinal motility is an important factor in maintaining the body's normal digestive function, manifested as the rhythmic contractions of gastrointestinal slow wave-driven movement, any impact on normal gastrointestinal contraction and relaxation of the factors that can lead to gastrointestinal motility disorders. Interstitial cells of Cajal (Interstitial cells of cajal, ICC) is the the gastrointestinal basic electrical rhythm (basic electric rhythm, BER) pacing and regulation, gastrointestinal autonomic nervous system and smooth muscle intermediary gastrointestinal movement and regulation the key to the pathogenesis of many gastrointestinal motility disorders and gastrointestinal tumors. Therefore, research the ICC pacemaker potential generation and regulation mechanism is very important. ICC intracellular endoplasmic reticulum calcium stores Ryanodine receptor potential (Ryanodine receptor, RyR) and inositol trisphosphate receptor (Inositol 1,4,5-trisphosphate receptor IP3R) receptor-dependent calcium channel in the ICC pacing play an important role in the production process. Endoplasmic reticulum calcium store release of ion channels and adjust the mechanism to become a research hotspot. Dachengqitang pass in the capture behalf of prescriptions, have a significant effect in the treatment of gastrointestinal motility disorders diseases. Emodin honokiol its main medicinal components involved in the regulation of the mobilization of intracellular calcium stores. Objective: the observed Dachengqitang active ingredients emodin honokiol pharmacokinetics in Wistar rats, the establishment of a Wistar rats fed Dachengqitang animal drug-containing plasma lyophilized powder preparation. And to investigate the drug-containing plasma of interstitial cells in the rat gastrointestinal Cajal (ICC) trisphosphate receptor (IP3R) mRNA expression Dachengqitang animals. Methods: RP-HPLC method for the determination of the two components of Wistar rats fed Dachengqitang liquid emodin, honokiol period animal tissue and body fluids drug concentration and 3P97 pharmacokinetic software in the plasma pharmacokinetic parameters. Preferred on the basis of the dynamic monitoring of the plasma concentration curve sampling time, collecting the separated plasma preparation of drug-containing plasma lyophilized powder, RP-HPLC detection efficiency of the active ingredient saved. Preparation of Large Chengqi Decoction animal drug-containing plasma, role in Wistar rat jejunum ICC cells, RT-PCR detection IP3R receptor mRNA expression. Results: The method can be well separated the two components emodin and honokiol concentration - chromatographic response linear correlation coefficients were gt; 0.999, average recoveries gt; 90%; two components mice metabolic elimination kinetics two-compartment model with first Tmax were 1.38h and 0.27h T1/2β to 30.68h and 6.68h T1/2ka 0.65h and 0.093h; plasma protein binding rate were 74.56 % and 65.63%. RP-HPLC detection confirmed the long-term preservation of animal plasma freeze-dried powder cryopreservation of animal plasma in active ingredient content was significantly increased (P lt; 0.05) lower temperature. Type 3 IP3R mRNA the rat jejunum ICC cells were expressed drug-containing plasma group than in the blank medium group increased significantly (P lt; 0.05), high concentrations of lower concentration group were significantly different (P lt; 0.05). Conclusion: single dose orally dachengqi granules, emodin with magnolol better absorption into the body is mainly distributed in the gastrointestinal, hepatic metabolism and renal excretion after entering the loop. Exist enterohepatic circulation. Animal plasma lyophilized powder Dachengqitang than traditional animal drug-containing plasma in the content of the active ingredient to maintain that there is a significant advantage. Animals Dachengqitang drug-containing plasma can significantly enhance the rat small intestine ICC IP3R mRNA expression, and presence of a dose-dependent manner.

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