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Study on Extraction and Antioxidation Synergistic Effect of Lycopene

Author: TaoZuoZuo
Tutor: QiuWeiFen
School: Nanjing University of Finance and Economics
Course: Of Food Science
Keywords: Lycopene Microwave-assisted extraction Extra high voltage Antioxidation Synergies
CLC: TQ611
Type: Master's thesis
Year: 2010
Downloads: 365
Quote: 0
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Abstract


Lycopene is a natural function of the carotenoids, anti-cancer, enhance immunity, anti-aging and other health functions, more and more people's attention. This paper studies the use of microwave-assisted extraction and high pressure treatment, the crude product was extracted from fresh tomato lycopene, further purification of the crude product routings and conditions, and to study the lycopene lycopene and other antioxidants on edible oil antioxidant and synergies. Adoption of saponification - dehydration - microwave-assisted extraction - recrystallization process fresh tomato lycopene extract, compared with the the centrifugal dehydration and vacuum drying, ethanol dehydration less loss of lycopene, while increasing tomato red Su purity. Helps to remove fat-soluble impurities in the tomato treated with 0.5M NaOH solution was saponified, the optimal time of saponification 3H processing after extraction of lycopene than the the unsaponifiables sample extraction rate increased 76.3%. By response to the face of microwave extraction of fresh tomato lycopene process optimization, quadratic regression model to establish the response value between the factors, resulting lycopene microwave extraction process as follows: microwave power 500W, microwave time 160s, liquid ratio of 8.2 mL / g, extracted three times the extraction of lycopene was 3.047mg / g dry matter, microwave power and liquid ratio have a significant impact on the extraction of lycopene. Compared with a solvent extraction method, the extraction time is shortened to reduce the use amount of the solvent. The results show that the microwave method is a fast and efficient extraction of lycopene development prospects. Use of ultra-high pressure solvent extraction of lycopene extracted from tomato powder, Exploration and process conditions, by studying the impact of various factors on the extraction of lycopene, to determine the optimal process conditions: room temperature, extraction solvent oil ether: ethyl acetate (1:1) mixed solution, liquid ratio to 30 ml / g, the processing pressure to 500 MPa, the dwell time for 300 s, this condition lycopene extract the best extraction rate of 3.047 mg / g. Compared with other commonly used extraction method, high pressure solvent extraction is a fast and efficient extraction of lycopene in tomato powder. Evaluation of the oxidative stability index (OSI) is added to edible fats lycopene and other antioxidants antioxidant activity and synergy. Rapeseed oil (0 to 0.0050% of added amount within the range) and lard (within the range of the added amount of 0 ~ 0.0025%) OSI value increases with the increase of adding amount of lycopene and lycopene lard antioxidant is better than rapeseed oil; compared to chemical synthetic antioxidants, lycopene antioxidant capacity in rapeseed oil is better than BHA, BHT, lard is better than BHT; compared with natural antioxidants, Both lipid system lycopene and tea polyphenols antioxidant capacity equivalent to better than alpha-tocopherol in rapeseed oil. alpha-tocopherol in rapeseed oil antioxidant lycopene has no effect, but with lycopene complex lard system works well, 0.0025%, 0.0050%, 0.0075% of alpha-tocopherol and 0.0025 % of lycopene compound used to make lard oxidation induced prolongation 63%, 130%, 167%. Tea polyphenols on antioxidant lycopene in Rapeseed Oil synergy is not obvious, the three concentrations of rapeseed oil oxidation induction period extended by 10%, 11%, 22%, and lycopene in lard system Synergistic effect, 0.0025%, 0.0050%, 0.0075% polyphenols and 0.0025% lycopene complex, to make lard oxidation induction period extended 49%, 120%, 122%. Synergies citric acid and EDTA on lycopene antioxidant rapeseed oil and lard and lard is better than rapeseed oil, may be considered as lycopene antioxidant synergist.

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