Dissertation > Excellent graduate degree dissertation topics show

Study on Breeding of Avilamycin Producing Strains and Conditions of Fermentation

Author: LiZheng
Tutor: TaoXingWu
School: Wuhan Polytechnic University
Course: Microbial and Biochemical Pharmacy
Keywords: Avilamycin Ferment Surfactant Protoplast fusion Strain selection The production green streptomycin Tü57
CLC: TQ465
Type: Master's thesis
Year: 2010
Downloads: 75
Quote: 0
Read: Download Dissertation

Abstract


Avilamycin, also known as avilamycin, the oligosaccharides ADM family of antibiotics as feed additives used in animal husbandry can prevent diarrhea in livestock, improve survival, and improve feed conversion rate, has obvious fattening and growth-promoting effect. This article from its physical and chemical properties, strain mutation screening methods, medium and fermentation conditions. In this experiment the the production green Streptomyces Tü57 (Streptomyces viridochromogenes Tü57) as the starting strains positive mutant screening avilamycin A content index components. We first starting strain by UV mutagenesis and chemical mutagenesis techniques mutagenesis original and screened to meet the requirements of the forward mutant strains, the avilamycin A production increased by 8.62mg / L to 15.26mg / L. Then, on this basis, multiple rounds of recursive protoplast fusion technique, the obtained mutants were screened through different channels recombinant fusion mutants obtained to further improve the yield, the yield increased by 15.26mg / L to 52.62mg / L. In the process of screening mutants, the paper attempts to use a thin layer - UV spectrophotometry detection Avilamycin of content screening. The Act with methylene chloride - methanol (9: 1) as a developing solvent, in the the HF254 spotting Exhibition layer silica gel plates, scraping effective spots, methanol dissolution, set UV 212.0 nm absorbance was measured with a standard curve control its content, the working curve is obtained a good linear relationship in the range of 10 to 60μg/ml, R2 = 0.9988, the average recovery was 100.5%. The method is simple, rapid, accurate, especially suitable for high-yield strain Avilamycin large-scale screening of fermentation liquid Determination. Completion of the high-performance liquid chromatography screening of high-yielding strains, strains rescreening. According to the principle of the Avilamycin cells, added to the culture medium macroporous resin X-5 and surfactant Tween-80, the fermentation broth Avilamycin try macroporous resin, the use of surface active agents to change the permeability of the cell membrane, reducing the negative feedback inhibition of metabolic synthesis of avilamycin, to improve the the fermentation gas-liquid state of the surface as well as the function of the fluidity of the fermentation broth, in order to improve the yield of antibiotic fermentation. The results show that the medium supplemented with 6% the macroporous resin X-5 and 0.3% Tween-80 can significantly improve the fermentation production of antibiotics. The strain on the fermentation yield is further increased by 52.62mg / L to 87.14mg / l.

Related Dissertations

  1. Adsortions of Dye by Modified Activated Carbon and Heavy Metals by Rice Husk-Based Activated Carbon,X703
  2. Activition of Surfactant Protein A and D in Aspergillus Fumigatus-Induced Innate Immune Responses in Human Cornea Epithelial Cells,R772.21
  3. Synthesis and Properties of Functional Waterborne Polyurethane Coatings,TQ633
  4. Low- diacetyl of Beer Yeast Mutants,TS262.5
  5. Dissertation for Master’s Degree Fujian Agriculture and Forestry University,S646
  6. Fermentation, Isolation, Purification and Structural Identification of Antimicrobial Substances Produced by Brevibacillus Brevis XDH,Q93
  7. Study on Interaction between Ionic Liquids and Surfactants & Application Research in Enhanced Oil Recovery,TE39
  8. Isolation and Breeding of ε-poly-L-lysine Producing Strain and Enhancement on Production Process,TQ922.3
  9. Study on the Screening and Enzymatic Properties of a Noel Cold-adapted Amylase-producing Strain from Alpine Frozen Soils in the Tianshan Mountains,TQ925
  10. Research on Low-Temperature Liquid Methanol Synthesis Catalyst,TQ223.121
  11. Pleurotus eryngii breeding and cultivation of Cell Engineering Research,S646
  12. Development of New Germplasms Resistant to Ralstonia Solanacearum by Protoplast Fusion of Diploid Potato,S532
  13. A Bacillus amyloliquefaciens production of antifungal substances,S476.1
  14. Studies on Isolation, Identification and Agricultural Activity of Symbiotic or Epiphytic Actinomycetes from Marine Fish,S476
  15. Preparation and Characterization of Porous SiO2 Microspheres,TB383.4
  16. Experimental Research on the Gel & Surfactant (Polymer/Surfactant Compound System) Alternating Injection Flooding after Polymer Flooding,TE357.4
  17. Study on LaMadian Oilfield Polymer after the Experiment of Foam Flooding,TE357.46
  18. Mutation and Screen of Aureobasidium Pullulans and Fermentation Technology,TQ920.1
  19. Typical Reversible Surfactants: Synthesis, Characterazation and Application in the Solubilization of LNAPLs,TQ423
  20. The Feasible Study of Improving Production and Injection by Water Flooding in Extra-high Water Cut Period,TE357.14

CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > Antibiotics manufacture
© 2012 www.DissertationTopic.Net  Mobile