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With the improvement of people's living standards, health consciousness is growing beverage needs of consumers is not simply the pursuit of branding, packaging, taste, and more emphasis on the health effects. Kudingcha in China more than two thousand years of drinking, medicinal history, long-term consumption, medicinal practice proved that it has heat and thirst, anti-inflammatory and detoxifying, promoting blood circulation, lowering blood pressure, laxative, pure heart and eyesight and other effects. Ilex new product development is very active, as raw material to develop a functional beverage with Ilex reported that there are many, but with Ilex as raw material to develop health carbonated drinks reported no. Carbonated drinks with other beverages are not the essential characteristics: refreshing, thirst-quenching, brake mouth, cooling. Thicker the leaves Kudingcha cuticle, the active ingredient of the dry tea amino acid, polyphenols, flavonoids and carbohydrates are usually surrounded by cell walls, the leaf Kudingcha the active ingredient diffusion leaching hindered by the cell wall, general extraction method , the unfit Kudingcha carbonated drinks production. In this study, cellulase, pectinase and papain the Ilex enzymatic extraction experiments, the Ilex carbonated drinks production enzymatic extraction process, to determine the optimal enzymatic hydrolysis of Ilex conditions and Ilex carbonated drinks main agent deployment program. The single cellulose enzyme extraction Kudingcha the the most suitable conditions: 30-40min, temperature 45-50 ° C, pH 5.5, enzyme concentration of 0.1-0.3%. Single papaya protease extraction Kudingcha the optimum conditions: 40-50min, temperature 55 ° C. pH 6.0 and 0.4 to 0.5% of the enzyme concentration. The single pectinase extraction Kudingcha the optimum conditions: 30-50min, temperature 45-50 ° C, pH 5.0-6.0, 0.2-0.3% of the enzyme concentration. Enzyme combinations optimum extraction conditions: 40-50min, temperature 55-60 ° C, pH 5.5-6.0; enzyme combination is a combination of cellulase, pectinase and papain. The results showed that single cellulase single papain, Single pectinase leaching effect are better than no enzyme leaching effect, and the enzyme composition of the leaching effect of sound on a single enzyme leaching effect, therefore, the main agent in the production of Ilex carbonated drinks, the combination of the application of enzyme extraction process. By regulating the addition amount of sucrose in the main agent, sweeteners and acidulants, identified by the recipe of the deployment of the main agent: 5% sucrose, 0.01% aspartame, citric acid 0.15%. Click the formula obtained Kudingcha carbonate drinks, sweet and sour taste of coordination, color Huang Liang, aroma, coordination. The proposed Ilex carbonated drinks enzyme extraction process, not only to improve the content of the active ingredient in the drink, but also avoids the conventional hot water extraction process is prone to easy oxidation, bad odor, precipitate, discoloration and other shortcomings, has obvious advantages in the production of Ilex carbonated beverages.
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