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Effect of Magnetic Fe3O4 Particles and Study of the Cellulase Immobilized by Magnetic Gelatin on Enzymatic Hydrolysis of Sunflower Seed Hull

Author: ChenLing
Tutor: YuanXingZhong；ZengGuangMing
School: Hunan University
Course: Environmental Engineering
Keywords: Magnetic Fe3O4 Cellulase Immobilized Enzymatic hydrolysis Sunflower shell Magnetic field
CLC: TQ223.122
Type: Master's thesis
Year: 2009
Downloads: 58
Quote: 1
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Abstract


Lignocellulose to produce fuel ethanol, the choice of more cheap, easy to collect the processing of raw materials, improve the enzymatic hydrolysis rate of the pretreated material was sugar rate, is one of the very effective ways to reduce production costs. Cellulase important role and position in the process of biomass conversion Needless to say, trying to improve the cellulase enzyme activity is also one of the most important ways to reduce the cost of production. Therefore, in this research project, intended for use in sunflower seed shells as raw material, the cellulase study, the magnetic Fe 3 O 4 and free cellulose enzyme The mechanism of the bio-magnetic effect as a guide to explore magnetic Fe 3 O 4 cellulase enzyme hydrolysis, and at the same time improve the vitality of the cellulase main research so as to achieve the purpose of reducing the cost of production of cellulase. The main research content of this paper is magnetized after Fe in 3 O 4 particles as an additive investigated in the process of enzymatic hydrolysis of sunflower seed shells, cellulose the enzymatic activity of the reducing sugar concentration, cellulose conversion, the influence of surface tension and pH of the hydrolysis solution, and role. In addition, magnetic Fe 3 O 4 particles as a carrier, to study the nature of the enzymatic hydrolysis of magnetic immobilized enzyme adsorption of cellulase immobilized gelatin embedding , and the addition of surfactant the magnetic immobilized cellulase activity, and preliminary analysis and elaboration of the reasons for these changes. The research results show that the magnetic Fe 3 O 4 dosage of 0.5 g / L 2 .0 g / L free cellulose enzyme After 48 h, the conversion rate of the increase in the concentration of the enzyme activity increased, reducing sugar and cellulose showed a more significant role in promoting. When the magnetic Fe 3 O dosage 2 g / L, 60 h cellulase enzyme activity can be increased to a maximum of 25.9%. Dosage 1.5 g / L of reducing sugar concentration from 6.950 mg / mL to 8.775 mg / mL; cellulose highest conversion rate of 60.531%, significantly higher than that do not add Fe 3 O 4 samples of cellulose conversion rate of 47.932%. The graded Add the the magnetic Fe 3 O 4 enzymatic activity stability is better than first added. Compared with the free cellulase under the same hydrolysis conditions, the magnetic immobilized cellulase capable of reducing sugar in the highest yield from 8.775 mg / mL increased to 9.162 mg / mL, compared to a 4.4% increase. Especially after the surfactant is added, the reducing sugar yield the highest value of up to 9.975 mg / mL, compared to a 13.68% increase.

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CLC: > Industrial Technology > Chemical Industry > Basic Organic Chemistry Industry > The production of aliphatic compounds ( acyclic compounds) > Aliphatic alcohols (alcohols, hydroxy compounds) and its derivatives > Aliphatic alcohol > Fatty Alcohols > Ethanol ( alcohol )
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