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Research on the Role of Rab5 and Rab7 in Cross Presentation

Author: LiuNa
Tutor: WuYuZhang
School: Third Military Medical University
Course: Immunology
Keywords: Red fluorescent protein Cross - submissions Protein expression Rab proteins Mutant
CLC: R392
Type: Master's thesis
Year: 2006
Downloads: 89
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Abstract

Cross-presenting foreign antigens are MHC-Ⅰ molecules restrictive submissions process, it differs from the classical MHC-I molecules presenting ways, presenting classical MHC pathway, the endogenous expression of the protein by proteasome After digestion, a small part of the degradation of peptides by TAP into the endoplasmic reticulum, the formation of the newly generated MHC-I molecules in the endoplasmic reticulum complex is transported to the plasma membrane, opposite MHC-Ⅱ molecules presenting the route of the main process proceeds endocytosis foreign antigens, such as MHC-Ⅰ and MHC-Ⅱ molecules in charge of the different sources of intracellular and extracellular antigens. Body case, DC cells are the main cross-presenting cells, infected cells endogenous antigen, then migrate to the lymph nodes obtained through the periphery of MHC-Ⅰ molecules formed peptide complex activation of antigen-specific cells cytotoxic T cells, the antigen source (cell, but a different cell, or vaccine foreign antigen) in this process is different from the classical MHC-Ⅰ molecule presenting pathway (in the antigen presenting cells synthesized antigen). Accordingly, in the above antigen-presenting antigen degradation and peptide delivery mechanism is likely to significantly different. In recent years, due to recognize the important role of cross submissions vaccination, its mechanism of causing widespread concern. The intracellular membrane protein transport is usually required to contain Rab proteins, protein complexes, Rab protein is a the events key molecule of eukaryotic membrane transporter. The total human genome contains about 70 Rab and Rab-like protein, they are usually located in a different area of ??membrane structure guanylate dependent switch mechanism regulating membranous transport four steps: vesicle budding generation, transport of vesicles vesicles tied tied and fusion of the vesicles with the target membrane structure. Existing evidence to suggest the several protein in the early sorting and the back transport vesicles (Rab4, Rab5, Rab11, Rab18, Rab22 andRab25), while Rab7 and Rab9 positioning the to late endocytic body has antigens in dendritic cells presenting, there is no research Rab molecules, therefore, this paper attempts to research dendritic cells. The high expression in dendritic cells by transfection Rab5 Rab7 mutant Rab5 Rab7 observed associated with the vesicular transport Rab molecules, and their mutants, to understand the intersection of the antigen presenting occurs mainly in the phagosome what stage of the maturation process. The first to use the Rab5 suppression to mutant Rab5S34N and Rab7 inhibition of mutant Rab7T22N observed two protein antigen cross-presenting results show that Rab5S34N cross inhibit antigen presenting, but or antigenic cross Rab7T22N of delivery was no impact. Read analysis Rab5 role in this process, we have constructed a Rab5 the activation mutants RabSQ79L results show that the antigen presenting effect Rab5 wild type consistent, indicating that early endosome antigen cross presenting main place, the results of this study for further analysis the transshipment and regulation of vesicles in the process of presenting antigenic cross to lay the foundation.

Research on the Role of Rab5 and Rab7 in Cross Presentation

Abstract

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