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Hepatoprotective effect of extracts of Sambucus

Author: YangWei
Tutor: WangMinWei
School: Shenyang Pharmaceutical University
Course: Pharmacology
Keywords: Sambucus Active site Ursolic Acid LC-MS Sambucus particles Hepatoprotective effect Immune system
CLC: R285
Type: Master's thesis
Year: 2005
Downloads: 209
Quote: 2
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Abstract


Study civil commonly used anti-hepatitis medicine Sambucus as the research object, AST, ALT, and pathological examination of the indicators observed a different solvent prepared extracts of Sambucus CCl 4 -induced acute hepatic injury, 75% ethanol extraction solvent system solvent extraction and adsorption of macroporous resin purification established Sambucus 75% ethanol extract by macroporous resin purification 30% ethanol eluate (LY) as the main active site, and preliminary pharmacodynamic study, the results showed that: LY carbon tetrachloride (CCl 4 ), D-galactose amine (D-Gal), concanavalin ( ConA)-induced acute liver injury showed good anti-liver injury, the dose-dependent; significantly improved normal mouse monocyte - macrophage phagocytosis, increase foreign body serum clearance rate, improve liver and spleen Network like endothelial system phagocytic index, suggesting LY enhance the role of the non-specific immune function of normal mice. As reported ursolic acid (ursolic acid) is the Miss British the hepatoprotective JiangMei the main active ingredient, we LY pharmacodynamics of the ursolic acid on CCl 4 , D- hepatoprotective effect of GalN-induced acute liver injury, the result table Ming Wusuo acid standard oral administration is not anti-liver injury. To this end, we further establish the LC-MS analysis method determination of ursolic acid in rat blood comparative study rats were given Sambucus extract and ursolic acid reference solution is equivalent to the same amount of Pharmacokinetics, ursolic acid was measured blood concentration - time curve, calculate the appropriate pharmacokinetic parameters. Ursolic Acid Rats were given standard rat plasma almost undetectable ursolic acid and give Sambucus extract the Hou Wusuo acid in about 1h peak, the peak concentration C max 294.8ng · mL -1 , the elimination half-life t 1/2 for 4.3h. Sambucus chemical composition and ursolic acid interactions can be speculated that promote the absorption of ursolic acid, can significantly affect the ursolic acid pharmacokinetic behavior in rats. Pharmacodynamics Preliminary identified 30% ethanol 75% ethanol extracts of Sambucus ethyl acetate layer by macroporous resin purification \we with the Sambucus particles (LYKL) for the control system hepatoprotective effect of LY Sambucus particles. Experimental results show that: LY and Lu Ying particles the CCl 4 , D-Gal, ConA thioacetamide (TAA) induced acute liver injury has a protective effect, can significantly reduce the various chemical The the substance caused acute hepatic injury serum ALT, AST increased, LY dose-effect relationship;, LY Sambucus particles could alleviate TG CCl 4 poisoned mouse liver tissue The accumulation and decreased MDA content, increase CCl 4 poisoning mouse liver T-SOD activity and total antioxidant capacity (T-AOC), also enhanced the CCl 4 the the poisoned mouse liver cell membrane and microsomal membrane Ca 2 -ATPase activity, reduce GSH compensatory increased degree of; the pathological results also show that the LY and Lu Ying particles significantly protect the liver cell damage The role; and LY role is significantly stronger than Sambucus particles. Meanwhile, LY Sambucus particles also against D-galactosamine hydrochloride induced acute liver injury caused serum ALP, TBIL, TG, NEFA levels and drastically reduced Glu content also significantly against D- galactosamine hydrochloride induced acute liver injury and liver tissue of liver glycogen content, Na -K -ATPase, Mg 2 -ATPase the of Ca 2 -ATPase the ca 2 -Mg 2 -ATPase activity reduced the role of LY was significantly stronger than the the Sambucus particles, and LY can inhibit this rat model of SDH activity decreased. Chronic liver injury experiments, LY can improve liver function and reduce animal mortality, the Sambucus particles can improve liver function to some extent, but not significantly reduce animal mortality, and mortality is greater than in the high-dose group dose group; LY and Lu Ying particles can to some extent reduce the composite factor induced cirrhosis serum LN, P Ⅲ P, HA and liver homogenates HYP content increased in biopsy can clearly see the LY and Lu Ying particles dose improved to varying degrees, liver pathology group compared with model group shows hepatoprotective effect, and there is a certain role in the inhibition of hepatic fibrosis the LY's stronger than Sambucus particles; antioxidant capacity detected that LY and Lu Ying The particles could improve the antioxidant capacity of the liver tissue, LY can enhance this model liver tissue Ca 2 -ATPase activity. Of liver disease caused by the LY and Lu Ying particles better against guinea pigs with autoimmune hepatitis model, both efficacy similar. Jaundice choleretic test results show that the the LY Sambucus particles choleretic role poor efficacy of jaundice. Test results show that the role of the immune system LY Sambucus particles improve LY induced immunodeficient mice reticuloendothelial function; improve HY induced immunosuppressive role of humoral immune; fetal spleen lymphocyte transformation test, The test results show that the CTX Sambucus particles can promote the added value of normal fetal spleen lymphocytes, ConA and LPS-induced fetal spleen lymphocyte transformation experiments, LY shows a certain extent, the Sambucus particles, there was no impact. In summary, 30% ethanol that Chinese medicine Sambucus 75% ethanol extract by macroporous resin purification eluate (LY) has better hepatoprotective effect, there is a certain role in the inhibition of hepatic fibrosis, and significantly better than the listed preparations of Sambucus particles, which may be related to stabilize liver cell membranes LY active ingredients, anti-lipid peroxidation and enhanced the antioxidant capacity of the liver tissue, improve liver cell membrane and microsomal membrane ATPase activity, improve liver the mitochondria SDH activity, as well as two-way adjustment of immune-related.

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