Dissertation > Excellent graduate degree dissertation topics show

Antifungal Activity Assay of Chinese Traditional Herbs and Screening for Yeast Gene Mutations in Utilization of Non-fermentable Carbon Sources

Author: ZhaoJiong
Tutor: JiangLingHuo
School: Tianjin University
Course: Medicinal Chemistry
Keywords: Paper method Chinese medicine alcohol extract Candida albicans Antifungal activity Saccharomyces cerevisiae Non-fermentable carbon source Gene deletion strains screened
CLC: R285
Type: Master's thesis
Year: 2007
Downloads: 71
Quote: 0
Read: Download Dissertation

Abstract


Topic : Objective : To determine the reliability of dry paper method and wet paper method for screening antifungal activity of traditional Chinese medicine . Method : the most important human pathogenic fungus Candida albicans (Candida albicans) as a biological model activity measurement using dry paper method and the wet paper method of Chinese medicine crude extracts and essential oils against Candida albicans . Method on dry paper method and wet paper method to better meet the formula Z2 = A logQ - M , that is proportional to the logarithm of the square of the diameter of the inhibition zone and pieces of paper containing a dose and dry paper method and no significant difference between the wet paper method . We then measured with a dry paper method and wet paper method of the alcohol extract 27 kinds of traditional Chinese medicine and 29 kinds of plant essential oil extracts activity against Candida albicans , snow tea , heavy floor and cinnamon oil . significant inhibitory effect . Conclusion: wet paper method have not been reported , and for the complex solubility characteristics of crude extracts of traditional Chinese medicine , the wet paper method as long as the right way , can be used as dry paper method complement activity in traditional Chinese medicine to provide more evidence for screening . Topic : Objective: Screening of Saccharomyces cerevisiae use of non-fermentable carbon source gene . Methods: The first 384 -pin photocopying board photocopying Saccharomyces cerevisiae gene deletion mutant library in YPD, YPE glucose , ethanol and glycerol as the carbon source and YPG medium comparative gene deletion strains in complete medium YPD and non-fermentable carbon source culture yl YPE and YPG growth to identify the strain grown in the medium of the non-fermented carbon defects , and then the concentration gradient of these strains with dilution method further verify that they use non - fermentation carbon source of defects , and thereby determining not to use the non-fermented gene -deficient strain of a carbon source . Results: 172 YPE and YPG medium on , have demonstrated growth defect 16 is growing well and grown in YPG defective in YPE 35 is grown in YPG good growth on the YPE defective 's . Conclusion: the discovery of gene function , provide important information to study the metabolism of a carbon source for Saccharomyces cerevisiae clarify Saccharomyces cerevisiae Central Africa fermentation carbon utilization related metabolic mechanism .

Related Dissertations

  1. Endophytic Fungal Diversity and Their Secodary Metabolites of Eleven Dominant Plant Species in the Dry-hot Valley of Yuanjiang, Yunnan Province, China,X172
  2. Isolation and Identification of a Probiotic Strain Capable of Producing Glycerol and Optimization of Fermentation Conditions for the Strain,S823.5
  3. Construction of Engineering Strain Saccharomyces Cerevisiae Metabolizing Xylose,Q78
  4. Purification and Some Properties of α-Amylase Inhibitor from Avena Nuda,S512.6
  5. Oral epithelial cells were infected with Candida albicans after HBD-2 mRNA and protein expression in vitro,R780.2
  6. Structural and Functional Studies of Saccharomyces Cerevisiae Aldo-keto Reductase Gre2,Q936
  7. A Genre Analysis of the Method Section of English Journal Papers in Applied Linguistics,H315
  8. Purification of Galactooligosaccharides,TS201.2
  9. Local human body and upper body sweat rate determination,R33
  10. Synthesis and Antifungal Activity of Novel Triazole Compounds,R96
  11. Genotyping and Antifungal Susceptibility Testing of Candida Albicans from Patients with Atrophic or Erosive Oral Lichen Planus,R781.5
  12. The Impact of Estrogen on the Expression of HWP1 Gene of Candida Albicans,R711.3
  13. Cloning and Expression of Skp1 and Rav2 from Saccharomyces Cerevisiae in Escherichia Coli,Q78
  14. The Role of Saccharomyces Cerevisiae NADH Kinase Pos5p on the Defense of Oxidative Stress,Q93
  15. Biological Characterizations of the Mutant Strain with HOR2 Gene Deleted in Sacchromyces Cerevisiae,Q78
  16. An Investigation on the High PKA Activity and Heat Shock Resistance of Saccharomyces Cerevisiae,Q932
  17. Breeding of Indigenous Wine Yeast and Fermentation of Low Alcohol Wine,TS262.6
  18. Study on Antifungal Substance from Marine Bacillus Subtilis DM03,S476.2
  19. Genotyping of Oral Candida Albicans Isolated from HIV-infected Patients,R512.91
  20. The Resistance of Candida Albicans to Common Antifungal Drugs and Its Expression of CDR1 and CDR2 Gene,R440
  21. Study of Real-time Quantitative PCR on the Early Diagnosis of Deep-seated Candida Infections,R450

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile