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Bupleurum important medicinal plants, bulk herbs used in countries such as China, Korea and Japan. \medicinal Bupleurum the original plant. Bupleurum medicine to root, root contains of bupleurum saponins and volatile oil ingredients, has anti-viral, anti-bacterial endotoxin, anti-inflammatory, anti-tumor, anti-convulsant, antipyretic, blood fat and promote secretion, liver protection, improve immunity effect , high medicinal value. In recent years, the Bupleurum the market demand increases every year, and excessive mining in turn led to fewer wild resources. But planted Bupleurum low seed germination and cultivation techniques difficult. Origin Bupleurum there are different varieties mixed phenomenon, caused by the Bupleurum herbs germplasm impure, of varying quality, resulting in the decline in the overall quality of affect the medication the accuracy and stability of the pharmaceutical quality, but also impact to the Bupleurum the export. Existing has been accepted by the majority of Yaonong the commercial cultivation germplasm purification, rejuvenation, and encouraged of quality variation small high quality Bupleurum plants and mass production and supply market is an urgent problem. In order to establish the optimal selection of North Bupleurum EduCare technology system, mass production North Chaihu quality seedlings and seeds, this study on the cultivation of Shanxi Wanrong Chai Seeds of No. 1 North seed coat damage and plant hormone treatment trial compared germination rate; application of plant tissue culture technology for rapid quality planting Shanxi Lingchuan, Wanrong, Zuoquan, Ruicheng, Fangshan, Lingqiu Gansu Longxi and Shangluo 8 North Bupleurum Reproduction; application Spectrophotometric determination of transplanting six months after the test-tube plants root the of Saikkosaponins content, HPLC-UV method for the determination of its the Bupleurum saponins a and saikosaponin of d of content; determined by spectrophotometry, compared the test-tube plants, transplanting plants, and the first 2 years of the transplant field test tube plants, and pest and natural cultivation of leaf chlorophyll content. Experimental results show that: the plant hormone treatment of North Bupleurum seed germination no significant effect, polished the damage processing testa seed germination rate can be increased by 20% and significantly reduce seed germination time. Additional 6-BA 1.0mg / L and KT 0.2 mg / L of B 5 medium suitable for the rapid proliferation and growth throughout North Bupleurum buds; additional NAA 0.1mg / L, IBA 0.5 mg / L and DSC 1.0 mg / L of 1/2 MS medium on rooting of plantlets in North Bupleurum good role; plantlets of Bupleurum chinense years breeding coefficient over 1 × 10 8 , transplanting The survival rate of 94% to 97%. Plantlets were transplanted the Daejeon six months after the roots saikosaponin content to meet the quality inspection standard. North the Bupleurum test-tube plants in good condition; bud proliferation and transplanting stage growth and photosynthesis in vitro plants after transplanting nursery bowl ability.
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