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Bamboo is Gramineae Bambusoideae perennial evergreen plants a long edible and medicinal history in our country. Bamboo leaves rich in bioactive components such as flavonoids and polysaccharides, owing to its excellent anti-free radical, anti-oxidation, anti-aging, and cardiovascular protection of biological effects, has been widespread concern of the domestic and foreign markets. In order to improve the efficient use of bamboo leaves active component of this thesis through to establish bamboo leaves flavonoids and polysaccharides active component content detection methods, screening high content of varieties; focuses on bamboo leaves flavonoids and polysaccharides active component of efficient extraction, separation and purification its efficient preparation techniques, the following results were obtained: selection of spectrophotometry and high performance liquid chromatography, a comparative study of bamboo leaves flavonoids in differences, and to establish a quantitative measurement method. NaNO2-Al (NO3) 3-NaOH colorimetric method is more commonly used, but the high measurement results, to the orientin glycosides, vitexin as standard, the HPLC assay results are more reliable and accurate. 27 different varieties of bamboo leaves flavonoids content was measured too dry bamboo leaves flavonoids content of 0.50% to 1.72%, and that 5.0 ~ 17.2mg / g, with the literature [1] reported range agreement. Determination of the content of the different varieties of bamboo leaves polysaccharides have polysaccharide content between 1.94% to 5.69%, ie 19.4 ~ 56.9mg / g. Dan Zhu, bamboo, bamboo is widely distributed, with higher levels of flavonoids and polysaccharide extract of bamboo leaves flavonoids and polysaccharides active component in industrial raw materials; flavonoid content of water bamboo, the goose feather bamboo polysaccharide content is particularly high, but less distributed , not suitable for industrial raw materials. For different age groups of bamboo leaves, old leaves the film polysaccharide content was significantly greater than the newborn leaves piece; flavonoid content due to bamboo species there are obvious differences. Preferred by the single factor experiment and orthogonal experiment to determine the optimum extraction technology of bamboo leaves flavonoids. Water bath to extract the best conditions: temperature 50 ℃, extraction time 2.0h, ethanol concentration of 60% (volume fraction), the ratio of 1:20 (g: mL), the impact of various factors on the extraction rate order : solid-liquid ratio gt; ethanol concentration gt; temperature gt; time; ultrasonic extraction conditions: ultrasonic time 30 min, 60% ethanol, solid-liquid ratio 1:15 (g: mL), the various factors on the extraction rate The degree of influence as follows: extraction time gt; ethanol concentration gt; solid-liquid ratio. By comparison of several resins Preferably, the isolation and purification of the AB-8 resin; through static desorption experiment, 70% of ethanol effects better than the 90% ethanol, and desorption of ethanol better than methanol; resin column adsorption process the process of adsorption and desorption are gradation curve, with 10% to 90% ethanol desorbing gradient elution, 40% ethanol elution most flavone desorption column packed with an amount of 47.0mg (47.1cm3 volume). Bamboo leaf extract after a combination of film processing become clear and transparent, bright color; extract used in the test volume is about 90L extract concentration of 9.32g / L ceramic membrane, 7.08g / L ultrafiltration membrane, the membrane use in good condition and stable flow rate, concentration polarization phenomenon did not occur; the film processing flavonoids extract, and then the processing of resin and polyamide resin, polyamide purification effect was significantly better in the AB-8, 80% ethanol, flavonoids content can reach 40.35%; polysaccharide extract of bamboo leaves to test the water extraction, inorganic and organic film processing, ethanol precipitation and then by more than 85%, the polysaccharide content increased to 40.20%.
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