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Study on Relationship between Cystic Fibrosis Trans-membrane Conductance Regulator and Pathogenesis of Ovarian Hyperstimulation Syndrome

Author: TangRuiLing
Tutor: JinLei
School: Huazhong University of Science and Technology
Course: Obstetrics and Gynecology
Keywords: Cystic fibrosis transmembrane conductance regulator Ovarian hyperstimulation syndrome Chloride channel E2
CLC: R711.75
Type: Master's thesis
Year: 2007
Downloads: 40
Quote: 1
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Abstract


Objective: To establish the ovarian hyperstimulation syndrome (OHSS) rat model , a preliminary study of the cystic fibrosis transmembrane conductance regulator (CFTR) expression changes and significance of OHSS rat ovary . Methods : 22 -day-old minor Wistar female rats were randomly divided into conventional stimulation group and the over-stimulation group , OHSS model established Ujioka method . Measured serum E2 level , Meilan solution 30min after tail vein injection in peritoneal fluid and ovarian , peritoneal and ovarian vascular permeability were measured in peritoneal lavage fluid and ovarian tissue the Meilan content to determine with a spectrophotometer ; the comparison between the two groups of ovarian weight , serum E2 levels and ovarian , peritoneal fluid Meilan content , and respectively by immunohistochemical methods and Western blotting method to compare between the two groups CFTR protein expression changes in ovarian tissue . Results : After injection of hCG 48hrs hyperstimulation group rat ovarian weight , serum E2 , peritoneal fluid , and ovarian Meilan content was significantly higher than that of conventional stimulation group ; immunohistochemical methods detected CFTR brownish yellow particles expressed in the rat ovary luteal cell cytoplasm , immunohistochemistry and immunoblotting method for quantitative analysis display the hyperstimulation group ovarian CFTR protein expression levels significantly higher than that of conventional stimulation group . Conclusion: overdose PMSG hCG induced blood E2 levels abnormally elevated , a significant increase in the amount of CFTR expression in vivo , followed , presumably through the chloride channel and epithelial cells secrete hyperactivity mechanism , causing the liquid too much accumulation in the ovaries and the third cavities , etc. , so as to participate in OHSS ovarian enlargement and cyst formation and water , electrolyte imbalance pathophysiological changes .

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CLC: > Medicine, health > Obstetrics and Gynaecology > Gynecology > Other diseases of the female genital > Ovarian disease
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