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Background Chronic hepatitis is a serious hazard to human health, affecting human life expectancy and quality of life of the disease, is currently China and many other countries is one of the important disease burden. Therefore, the depth of chronic hepatitis pathogenesis, prevention and treatment of the disease has important guiding significance. Chronic hepatitis is influenced by genetic and environmental factors of multifactorial diseases, gene - gene and gene - environmental interactions in the pathogenesis of the disease has an important role. In recent years, with the human genome project and the rapid development of molecular biology technology, from the genetic level to clarify the pathogenesis of chronic hepatitis medical profession to become the focus of attention. Chronic hepatitis B is the most common chronic hepatitis main, alcohol intake is an important risk factor. Many studies have shown that alcohol is closely related to the damage and chronic hepatitis, and alcohol dehydrogenase 2 (ADH2) gene mutation Arg47His affect the metabolism of alcohol is also an important factor. Therefore, the variation and how the relationship between chronic hepatitis is a problem worthy of further exploration. Studies have shown that this mutation with alcohol dependence and liver cirrhosis, liver cancer are closely related. However, variations on the ADH2 gene Arg47His relationship with chronic hepatitis, as well as the variation and interactions between other risk factors for chronic hepatitis illness affects how, at home and abroad has not been reported. Objective 1 To explore the ADH2 gene Arg47His variation and the relationship between chronic hepatitis. (2) investigate the ADH2 gene Arg47His between mutation and other risk factors in the pathogenesis of chronic hepatitis interaction. Methods A hospital-based case-control study design, in order to confirm the diagnosis of chronic hepatitis patients in this study. Select Jinan Infectious Disease Hospital diagnosed 252 cases of patients with chronic hepatitis Han as case group and 223 Han healthy control group. Detected using PCR-RFLP method Arg47His ADH2 gene mutation. Measurement data using the F-test comparison between the groups mean difference; using x ~ 2 test analysis count data in each group the difference between the frequency distribution; using univariate and multivariate Logistic regression analysis ADH2 gene mutation associated with chronic hepatitis Arg47His relationships. ADH2 gene mutation and other factors Arg47His interaction between case-control and uncontrolled case studies, and are analyzed by Logistic regression. Results 1 sex, age, smoking in patients with chronic hepatitis group and the control group showed no significant difference between (P> 0.05). Drinking, family history of hepatitis, the difference between the two groups was statistically significant (P <0.05). Positive family history of alcohol and hepatitis chronic hepatitis ratio was significantly higher. 2.ADH2 gene variant genotype frequencies Arg47His chronic hepatitis group (GG, GA, AA frequencies were 13.1%, 48.4%, 38.5%) and control group (GG, GA, AA frequencies were 7.2%, 42.6%, 50.2% ) There was a significant difference (P = 0.014). Arg47His ADH2 gene variant allele frequency of chronic hepatitis group (G, A allele frequencies were 37.3%, 62.7%) and control group (G, A allele frequencies were 28.5%, 71.5%) there was a significant difference compared (P = 0.005). Wild-type genotype and allele frequencies were significantly higher in chronic hepatitis. 3.ADH2 gene mutation associated with chronic hepatitis Arg47His relationship to homozygous mutant AA as the reference group, logistic regression analysis to Arg47His ADH2 gene mutation associated with chronic hepatitis relationship. Mixed in before adjusting for potential factors, GA and GG genotype OR was 1.48 (95% CI :1.01-2 .17) and 2.38 (95% CI :1.24-4 .59). Adjusted for sex, age, smoking, alcohol consumption, family history of hepatitis, the OR was 1.46 (95% CI :0.97-2 .19) and 2.51 (95% CI :1.25-5 .02). 4.ADH2 gene Arg47His mutation analysis of the interaction between drinking and analyzed using case-control design, AA GA and GG genotypes into two levels, in order to analyze the logistic regression model between drinking prevalence of chronic hepatitis interaction . The results show that both before and after adjustment for potential confounding variables, the effects of two factors exist various factors are greater than the sum of individual effects exist. Before adjustment for potential confounding factors, effect index (S) of 1.90, interaction excess relative risk (RERI) was 1.62 percentage attributed to interaction (AP) was 36.64%. Adjusted for sex, age, smoking, family history of hepatitis, S is 2.08, RERI to 2.27, AP was 42.22%. 5.ADH2 gene Arg47His variation and the interaction between the level of HBV replication analysis of the case studies analyzed using uncontrolled. Will be divided into AA GA and GG genotypes two levels, and as the dependent variable, the level of hepatitis B virus replication as independent variables to be analyzed by logistic regression model, and the gender, age, smoking, drinking, Alt, Ast, Tbil, Dbil , A, G, Alp, Ggt as a potential risk factor to be adjusted. The results showed that, ADH2 gene mutation and hepatitis B virus replication Arg47His exist between the level of interaction between the multiplicative model, OR = 3.22, (P = 0.07). In AA, GA and GG genotypes as the dependent variable, and AA genotype as a reference group to the level of hepatitis B virus replication as independent variables, using multiple logistic regression model state to be analyzed, and gender, age, smoking, drinking, Alt, Ast, Tbil, Dbil, A, G, Alp, Ggt as a potential risk factor to be adjusted. GA genotype between the level of HBV replication with no significant interaction multiplicative model, the OR was 1.10, (P = 0.81); however GG genotype and hepatitis B virus replication levels exist between the multiplicative interaction the OR was 3.51, (P = 0.06). Conclusions 1. Smoking, sex, age, and no significant correlation with chronic hepatitis. The positive family history of alcohol and hepatitis were significantly associated with chronic hepatitis. 2.ADH2 wild-type gene variant gene Arg47His type may be China's Han pathogenesis of chronic hepatitis important risk factor. 3.ADH2 wild-type gene variant gene Arg47His types in between drinking the pathogenesis of chronic hepatitis significant interactions. 4.ADH2 gene Arg47His wild-type gene variant types and between the level of hepatitis B virus replication in chronic hepatitis, there are also a significant interaction. Significance and Innovation: In this study, case-control study design and uncontrolled cases at home and abroad for the first time a more comprehensive analysis of the ADH2 gene mutation associated with chronic hepatitis Arg47His relationships, and the variation and the interaction between other risk factors in patients with chronic hepatitis The effect of illness. The results not only for the further study of its kind to provide a reference basis, but also to identify high-risk populations with chronic hepatitis and chronic hepatitis prevention and control has certain guiding significance.
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