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Objective: To study Epimedium rat degenerative cervical bone tissue Ca and P metabolism and intradiscal IL-1β, IL-6 content of TNFα observed Epimedium inhibition of degeneration of the cervical spine, explore Longspur hopane prevention mechanism of cervical spondylosis. Methods: A 6-month-old SD rats 30, weight 220 ± 20g, male or female, after adaptive feeding, were randomly divided into the model group and the sham-operated group, model group 20, 10 sham-operated group. Model group cut through the neck muscle and supraspinous, interspinous ligament resection, the establishment of dynamic and static force imbalance rat model of cervical spondylosis; sham-operated control group was given the sham treatment. Beat cervical X-ray examination after January, March, according to the results of X-ray film confirmed after the establishment of the model, the model group was divided into the Epimedium model group (A) and saline model group (B) , respectively, of feeding Epimedium water extracts and saline sham control group (C) of feeding saline. Were taken after dosing the February rats serum and cervical bone tissue calcium and phosphorus content of Ca, P, ALP indicators and bone tissue in the serum samples were measured by automatic biochemical analyzer; take the rats cervical disc were detected by radioimmunoassay IL1-β, IL-6 and TNF-α content, and then the statistical analysis of the group of data between the specimens. Results: ① The postoperative January shoot the X-ray results show: the model group and the sham-operated group two sets of X-ray score was no significant difference (P> 0.05), while in March after X-ray prompt: cervical degeneration model group becomes clear that the two groups significantly different statistically significant (P <0.05), and in January the model group were significantly different (P <0.01), with a significant statistical significance. ② administration in February after the group A rat serum phosphorus and alkaline phosphatase levels than in group B and group C were significantly increased (P <0.01), with significant statistical significance. No significant difference between the three groups of serum calcium levels. (3) B group cervical bone tissue Ca content of P and C group compared to significantly lower, the difference was statistically significant (P <0.05), A group of cervical bone tissue Ca content of P and B group compared to the significantly increased (P <0.01), with significant statistical significance; A set of cervical bone tissue content of P and C group is not elevated, was not statistically significant (P> 0.05). ④ the B group cervical intervertebral discs IL-1β, IL-6, TNF-α content of group C compared to significantly higher, statistically significant (P <0.01); the A group jugular disc tissue IL-1β, IL -6, TNF-α content of group B compared to significantly lower, statistically significant (P <0.01), group C compared to the difference is not significant, was not statistically significant (P> 0.05). Conclusion: ① The surgical destruction of the the rat cervical physiological balance method to cause cervical instability modeling March establish animal models of degenerative cervical. ② Epimedium can increase the degenerative cervical bone tissue calcium and phosphorus content, inhibition of cervical degeneration. The ③ Epimedium can reduce the degenerative cervical bone tissue alkaline phosphatase activity, inhibit osteophyte formation, slow down the degeneration of the cervical vertebrae. The ④ Epimedium down degenerative cervical disc content of cytokines, presumably to prevent the inflammatory response and mitigation of disc degeneration. ⑤ cervical calcium and phosphorus reduction of cervical degenerative pathology results. ⑥ degenerative disc release cytokines can accelerate the degeneration of the intervertebral disc. ⑦ degenerative the rat cervical disc tissue IL-1β, IL-6, TNF-α content increased calcium and phosphorus content of bone tissue and cervical reduce, may be an important factor for cervical disease.
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