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The Interaction Mechanism of 1,10-Phenanthroline Complexes of Ferrum(III) and the Studies on Fluorescence Fiber-Optic DNA Biosensor

Author: WangShaoJuan
Tutor: NiuShuZuo
School: Qingdao University of Science and Technology
Course: Analytical Chemistry
Keywords: Iron - phenanthroline complexes DNA Fluorescence Optical fiber Biosensor
CLC: TP212.2
Type: Master's thesis
Year: 2007
Downloads: 113
Quote: 1
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Abstract


Deoxyribonucleic acid (DNA) is one of the most basic substance of life, widely found in a variety of in vivo and is stored, the main material basis of life processes of replication and transmission of genetic information. Metal ions and their complexes play a central role in many life processes, is an important probe of nucleic acids and other biological macromolecules. In this paper, for the purpose to study the DNA sensors, synthesis and characterization of two iron - phenanthroline metal complexes; fluorescence spectroscopy, UV visible spectroscopy, Electrochemical study of their interaction with DNA; using common bonding to the fiber surface modification, respectively to these two kinds of metal complexes made of a hybridization indicator DNA fluorescent optical fiber sensor, specifically as follows: (1) o-phenanthroline and the neutral derivative of imidazole and [f the phenanthroline hydroxyphenyl microphone wow [f] phenanthroline ligand synthesis [Fe (phen) 2 IP 3ClO 4 2 O (phen = Phenanthroline phenanthroline, IP = imidazo [f] phenanthroline phenanthroline) and [Fe (phen) 2 PHPIP] 3ClO to · 2H 2 O (phen = Phenanthroline phenanthroline PHPIP = p and Mia Wow [f] phenanthroline phenanthroline) complexes of the two metals; through complexes of infrared spectroscopy (IR), elemental analysis (EA) measured to determine the molecular composition of the complexes; with 3-D fluorescence spectroscopy verified their fluorescence characteristics. (2) the use of fluorescence spectroscopy, UV - visible spectroscopy and electrochemical methods to study [Fe (phen) 2 IP] 3 and [Fe (phen) 2 the PHPIP,] 3 with salmon sperm DNA interactions determine the role of party complexes with DNA binding ratio and binding constants were determined. The experimental results show that the [Fe (phen) 2 IP] 3 and the mode of action of DNA intercalation combined ratio of 1:1, the binding constant of 4.00 × 102L · mol -1 . [Fe (phen) the PHPIP,] 3 mode of action of DNA embedded plug combination combined ratio of 1:2, the binding constant of 1.02 × 103L0.5 · mol -0.5. (3) of fiber surface modification, covalent bonding to prepare DNA modified quartz fiber, each of the [Fe (phen) 2 IP] 3 and [Fe (phen) of 2 PHPIP] 3 for hybridization indicator made of a the DNA fluorescence fiber-optic, use them to detect and fixed on the fiber-optic probe DNA complementary target DNA. The experiments show that to [Fe (phen) 2 IP] 3 detection of complementary DNA hybridization indicator, the linear range of detection of 1.25 × 10-8 mol- L-1 ~ 1.50 × 10-7 mol · L -1 , a detection limit of 1.35 × 10-9 mol L -1 . [Fe (phen) 2 PHPIP 3 detection of complementary DNA hybridization indicator, the linear range of detection of 4.98 × 10-7 mol L -1 ~ 4.88 × 10-6 mol · L -1 , a detection limit of 1.08 × 10-7 mol · L -1 .

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