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Study on the Defining Method of Sensitive Alga Which Used in Algae Infrared Radiation Detecting Organic Toxicities

Author: DingYanYan
Tutor: ZhangZhi；GuoWeiHua
School: Chongqing University
Course: Municipal Engineering
Keywords: Algae infrared radiation Organic toxicity Sensitive algal determine method
CLC: X832
Type: Master's thesis
Year: 2008
Downloads: 49
Quote: 1
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Abstract


The algae while having photosynthetic phosphorylation in energy metabolism and oxidative phosphorylation system, change the energy of toxic substances toxic response is stronger than the other microorganisms. Algae cells in the life activity will generate heat radiation to infrared radiation to the outside, the heat generated when the cell population reaches a certain number of values ??in the form of infrared radiation may be infrared thermometer measuring. Can react according to the the algae solution temperature changes the algal the poisoned toxicity of response, the proposed use of sensitive algae algal test infrared test method to detect organic compounds. Sensitive algae is the temperature difference in the the organic toxic algae infrared test toxic response time is short, in response to many kinds of drugs, to low doses special algae. The existence of sensitive natural algae algae, how to determine algal infrared test key. To this end, exploratory study to carry out the method for determining the sensitive algae. Using portable infrared thermometer to detect organic chemicals caused by algae solution temperature changes, analyze the relationship between the of organic toxic algal infrared radiation from the test algae species to identify the obvious organic toxic response algae as sensitive algae; clear sensitive algae test the sensitivity range of organic chemicals; master sensitive algae growth characteristics analysis of the sensitivity of the different growth stages algae; secondary two-factor regression general rotation of six kinds of the binary organic chemicals joint toxicity study, the following conclusions: 1) the heat generated by the algae cells in the activities of life to the infrared radiation radiated to the outside world, when the number of the cell population reaches a certain value, the heat generated by the form of infrared radiation infrared thermometer determination. The algae while having photosynthetic phosphorylation in energy metabolism and oxidative phosphorylation system, change the energy of toxic substances toxic response is stronger than the other microorganisms. 2) the response of algal organic chemicals, the average maximum temperature difference of 0.11 ° C to 0.27 ° C, the response time of 3.0min ~ 4.8min, the significant difference in response between the algae species. 3) temperature response, time to response, response to drug evaluation basis, initially identified Selenastrum capricornutum sensitive algae in the test algae. Selenastrum capricornutum to 10 kinds of organic chemicals in response to the absolute temperature of from 0.17 to 0.5 ° C, the average temperature of 0.27 ° C, absolute maximum temperature difference between the time average time immediately ~~ 9.0min 4.4min. 4) the Selenastrum capricornutum measurement sensitivity of typical organic light-emitting bacteria sensitivity. Ultimately determine the Selenastrum capricornutum sensitive algae in the test algae. 5) Selenastrum capricornutum develop initial chlorophyll content 0.35mg / L, in the logarithmic phase; 13-14D in the stable phase at the beginning of the 1-2d in the delayed phase, 3-12d; after 15 days in the death of. The Selenastrum capricornutum organic toxic for algae infrared radiation in the best period of logarithmic growth phase. 6) phenol, toluene, benzene, chlorobenzene, 4 kinds of organic pharmaceutical composition, a mixture of phenol and toluene two organic Drug Interactions additive effect. A mixture of toluene and chlorobenzene the two organic Drug Interactions synergistic effect. Benzene and phenol, benzene and toluene, benzene and chlorobenzene, phenol, and chlorobenzene mixture, joint effects mainly antagonistic activity.

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CLC: > Environmental science, safety science > Environmental Quality Assessment and Environmental Monitoring > Environmental monitoring > Water quality monitoring
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