Dissertation > Excellent graduate degree dissertation topics show

Screening Molecular Targets of Phytophthora Sojae RXLR Effectors

Author: HuangZuo
Tutor: WangYuanChao
School: Nanjing Agricultural College
Course: Plant Pathology
Keywords: Phytophthora sojae RXLR effectors hypersensitive response yeast two hybrid
CLC: S435.651
Type: Master's thesis
Year: 2010
Downloads: 8
Quote: 0
Read: Download Dissertation

Abstract


Phytophthora sojae (Kaufman & Gerdman) is an aggressive pathogen causing one of the most destructive diseases root rot on soybean,which is able to infect and kill soybean plants throughout much of the growing season worldwide. We bioinformatically identify almost 350 candidate RXLR effector genes in the genomes of Phytophthora sojae.These effectors can block plant disease resistance,improve the ability of pathogen infection and some effector proteins also has the eliciting function.Although their targets has not deeply researched.Current opinion on bacterial effectors’targets have been deeply researched,according to plant disease resistance signaling pathway is highly conserved,we cloned some researched bacterial effectors’targets,such as AtRin4, AtHSP90, AtSGT1b, AtRAR1, StR3a.By testing reporter genes in yeast two-hybrid system, we can find AtSGT1b and PsAvr1b-sp may interact with each other. Base on the above the findings, according to soybean’s genome sequence website and the NCBI website, we find 5 homology sequences with AtSG1b, GmSGT01 (Glyma01g43150.1)、GmSGT02 (Glyma16g29450.1)、GmSGT03 (Glyma09g23980.1)、GmSGT04 (Glymal0g37440.1)and NbSGT1.1.By using yeast two-hybrid technology again, GmSGT01, GmSGT03 may interact with PsAvr1b-sp.MAPK signaling pathway as a plant disease resistance signaling pathway has become an important part of various types of effectors’targets.We use potato virus vector PVX system, highly expressed a key kinase NbMKK1 (Nicotiana Benthamiana) of MAPK signaling pathway in tobacco cells can cause the HR response, but altogether expresses some effector molecules PsAvh331-sp, PsAvh94-sp and PsAvh328-sp may suppress the HR response which induces by it. By testing reporter gene and switch vector assay, we can find NbMKK1 and PsAvh94-sp may interact with each other. According to this research we conclude Phytophthora sojae effector Avh94 can block the signal pathway of plant immunity.

Related Dissertations

  1. Yeast Two-Hybrid Screening of 14-3-3-Interacted Proteins During Early Cotton Fiber Development,S562
  2. Comparison of Gene Expression Data Cluster Methods and Gene Network Construction for Phytophthora Sojae Genes,S435.651
  3. The Identification of the Avirulent Gene PsAvr3b in Phytophthora Sojae,S435.651
  4. The Investigation of Interreactions between the VirB4 Protein of Brucella and the Bovine Embryo Trophoblastic Cells,S852.61
  5. Screening of the Avian Influenza Virus PA Related Protein with Yeast Two-Hybrid Techniques,S852.65
  6. Preliminary Research of Function and Differentiation Regulating Mechanism of RAI 16 Protein Interacting with Tec Tyrosine Kinase,R73-3
  7. Screening for Proteins Interacting with Plant Pathogenic Oomycete RXLR Effector AVR1B,S432.4
  8. Genetic Analysis of an Arabidopsis Thaliana Mutant Susceptible to Phytophthora Sojae Infection,S432.1
  9. Mapping of the Proteins Interacting Domains of Chicken Anemia Virus,S858.31
  10. Study on the Interaction between PICK1 and α-SNAP,Q75
  11. Screening of the Proteins Interacting with Porcine Calsarcin-2 by Yeast Two-Hybrid Techniques,S828
  12. Studies on Population Genetic Structure of Phytophthora Sojae,S435.651
  13. Transcriptome Research and Founction Analysis of GPR in Phytophthora Sojae,S435.651
  14. Genetic Diversity of Phytophthora Sojae in Heilongjiang,S435.651
  15. Molecular Mechanism of A Zinc Finger Protein MsZFN from Alfalfa,S541.9
  16. Screening Interacting Proteins with Deinococcus Radiodurans Peculiar Protein PprI,Q93
  17. Screening Interacting Proteins of Deinococcus Radiodurans Dps-2,Q93
  18. Isolation, Identification and Pathotypes of Phytophthora Sojae from Anhui Province,S435.651
  19. Screening for New Binding Proteins Interacting with the Influenza Virus Nucleoprotein by Yeast Two-Hybird System,S855.3
  20. Construction of Yeast Two Hybrid cDNA Library and Expression Analysis of Translationally Controlled Tumor Protein in the Early Stages of TcLr19 under the Stress of Puccinia Triticina,Q943.2

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Economic crop pests and diseases > Oil Crop pests and diseases > Soybean pests and diseases
© 2012 www.DissertationTopic.Net  Mobile