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The study quality standards of fine felt wool honeysuckle

Author: YangMiao
Tutor: MaYuYing
School: Chengdu University of Traditional Chinese Medicine
Course: Pharmacognosy
Keywords: Fine felting wool honeysuckle Quality standards TLC of the HPLC fingerprint Chlorogenic acid
CLC: R282.7
Type: Master's thesis
Year: 2008
Downloads: 134
Quote: 0
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Abstract


Honeysuckle as commonly used in traditional Chinese medicine, detoxification effect is quite good. Fine felting wool honeysuckle Lonicera similes Hemsl mainstream varieties produced in Sichuan honeysuckle, received are set out in the \Line with the Administration of Traditional Chinese Medicine in Sichuan subject felting wool honeysuckle produced in Sichuan honeysuckle mainstream varieties of fine quality research \Produced in Sichuan the mainstream medicinal development and utilization of standardized planting to provide new resources to provide a scientific basis for the \The results are as follows: for the first time carried out a detailed study of the harvesting and processing methods for fine felting wool honeysuckle, and provide a scientific basis for the determination of appropriate harvest and processing methods. That fine felting wool honeysuckle optimum harvest period for the yellow and white open flowers, sunny morning from 9 to 11:00 harvested; processing methods steam fixing - drying method chlorogenic acid content in the highest, and the color is good, and the The drying temperature can be better controlled at around 90 ~ 110 ℃ Lonicera guarantee a fine felt wool quality. More detailed morphological and histological comparative study of the fine carpet hair honeysuckle and gray felt wool honeysuckle for the first time, to identify the main distinguishing feature on the appearance and organization structure. Original plant morphology, were identified from the leaf texture, hair, veins, Corolla surface coat so; medicinal properties, mainly surface glandular trichomes from the bud (Corolla), non-glandular hairs how many distribution with hygiene conditions were identified; microscopic characteristics, mainly from glandular hairs, how much non-glandular hairs, shape identification. For the first time established a microemulsion TLC identification method of of fine felt wool honeysuckle flavonoids. Through 11 different habitats fine felting wool honeysuckle microemulsion TLC identification samples 741 (Yibin) spots of 743 (muchuan), and other samples compared to a significant difference in performance in the absence of certain ingredients and amount on different. Of the fine carpet hair honeysuckle and gray carpet hair honeysuckle n-butanol extract fractions were compared at the same time, they are significantly different in composition, there are differences in the quantity of certain ingredients. HPLC fingerprint of the first fine felting wool honeysuckle herbs. By a comparative analysis of 12 batches of different locations, fine the felting wool honeysuckle fingerprint, South River, Dayi fine felting wool honeysuckle, high similarity, fine felting wool honeysuckle of Yibin, muchuan region on large map differences. With gray felt wool honeysuckle's HPLC fingerprint comparison found that they are great differences in the composition and quantity of ingredients. LC / MS analysis of the fine carpet hair honeysuckle HPLC fingerprint of unknown peaks and for the first time, the results showed that the the fingerprinting main peaks were chlorogenic acid and chlorogenic acid isomers . Examine the 12 batches of different origin and processing methods thin blanket honeysuckle herbs of impurities, moisture, ash and acid insoluble ash, heavy metals and harmful elements and organic chlorine pesticide residue amount, based on experimental data, combined with the actual production and the development of its limited standard: impurities not more than 5.0%; Moisture not more than 15.0%; Ash not more than 10.0%, acid insoluble ash Not more than 2.0%. Determination of heavy metals and harmful elements results showed that the only sample 741 (Yibin) excessive levels of arsenic and cadmium, based on the measurement results, combined with the actual production, initially developed its limits, and not more than five millionths lead, cadmium should not exceed 10 million three quarters, arsenic not more than two millionths of mercury should not exceed 2/10000000, copper and not more than twenty millionths. Determination of organochlorine pesticide residues combined the temperament spectrum associated instrument analysis results show that the BHC, DDT and PCNB was not detected in the samples. Determination of organochlorine pesticide residues, combined with actual production, the initial preparation of the fine felting wool honeysuckle organochlorine pesticide residues limits, HCH (BHC), not more than 2/10000000, DDT (total DDT) not too 2/10000000 PCNB (PCNB) not more than one ten-millionth. Comprehensive consideration of the solubility of the medicinal active ingredient and the leaching effect, established a fine felt wool honeysuckle extract assay for ethanol hot dipping method. Formulating its content limit shall not be less than 25%. Dozen batches of different locations fine felting wool honeysuckle herbs and two Pihui of of blanket honeysuckle Determination of chlorogenic acid, and for the first time on the fine felt wool honeysuckle luteolin and caffeic acid content determination, the results show that: fine felting wool honeysuckle chlorogenic acid content of up to 11.30%, the lowest 5.59%, averaging 8.61%; herbs and fine felting wool honeysuckle chlorogenic acid content higher than the measured gray felt wool honeysuckle herbs. Recommended the fine felt hairy honeysuckle chlorogenic acid content limits specified as not less than 5.0%. Luteolin and caffeic acid assay results show that its lower content of fine felting wool honeysuckle. Fine felting wool honeysuckle storage method for the first time, results show that with the extension of storage time, medicinal surface color will gradually darken, and prone to infestation. Its main influencing factors Herbs harvesting period, processing methods and packaging materials.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Chinese herbal medicines > Various types of medicinal herbs
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