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Porcine Circovirus Disease (Porcine circo virus disease) is one of the important diseases that cause serious loss of the pig industry. Disease virus is porcine circovirus (PCV), PCV-2 the clinical pathogenic virus, the virus caused by post-weaning multisystemic wasting syndrome as the main feature. In this experiment, the Kunming mice as subjects, different routes of transmission of porcine circovirus type 2 (PCV-2) study provides strong proof for the production of circovirus type 2 route of transmission. Vertical transmission study will test mice were divided into three parts: pregnancy 6-7d clean grade female rats after intraperitoneal injection of 0.1mL containing 10 × 103 TCID50 PCV-2 virus solution, as the source of infection, the part of the female stillbirth and miscarriage phenomenon tail - cut the PCR analysis confirmed infected with PCV-2 successfully, In order to understand the results of vertical transmission, taking a pregnancy 16d female mice were sacrificed, to remove intrauterine embryo using PCR to detect the presence of the virus; will give birth healthy suckling mice for the test group and remove unhealthy suckling mice, sub-day-old suckling mouse, necropsy test group, respectively, 14 days, 28 days, 49 days, 56 days, and set the blank control. At 0-day-old neonatal rat's stomach extracted milk clot was detected by PCR test result was positive. 14-56 day-old suckling mice whichever is the heart, liver, spleen, lung, kidney, thymus, brain, lymph nodes, thyroid, intestine, uterus / testicular virus detection, no significant day-old suckling mice during the inspection day-old clinical symptoms, but the autopsy found that specific lesions of neonatal rat lung hemorrhage hyperemia, lymph node hemorrhage swelling, the histopathology observed on lymphocytes, macrophages necrosis. PCR detection of PCV-2 virus distribution results in the test group neonatal mice offspring 14,28,49 days old can be found in the lymph nodes, thymus, thyroid, lung, brain, heart, liver, spleen, kidneys, intestines , uterine or testicular detects the distribution of the PCV-2 virus. First 56 days, the lymph nodes was not detected to the PCV-2 virus, established by the above results can be proved in mice as a model of the PCV-2 vertical transmission. Contact horizontal transmission is divided into two parts, the test mice successfully with PCV-2 infection the clean grade Kunming mice, nasal fluid, saliva, urine, feces, and take the PCR detection, has the presence of PCV-2, and confirmed its as a source of infection, direct infection trial (in the same column infection). Same column into a clean grade mice polyculture, male and female, polyculture 40d after setting the blank control group, no significant clinical symptoms in mice, on Polyculture mice were sacrificed, PCR detection of various organs, have the presence of PCV-2; pathological changes of lung hemorrhage hyperemia, bleeding swollen lymph nodes, we found that direct contact (with bar) PCV-2 infection in the rat population. The level of non-contact spread Kunming mice were randomized clean grade A cage into five have been infected with PCV-2 mice, B cage placed 10 meters of clean grade Kunming mice and in another room set G group of five blank control, the observed incidence position. 50 days later, the mice were no obvious clinical symptoms, then we B caged mice were sacrificed, the obvious symptoms of bleeding of the lungs of mice, lymph nodes and redness of the phenomenon, but a general lesions compared with the first two modes of transmission minor. And the lung, spleen, liver, lymph node PCR specific detection, are positive results. Airborne spread of non-contact level, PCV-2 still spread.
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