Dissertation > Excellent graduate degree dissertation topics show

Geometridae , Arctiidae some species of esterase isozymes

Author: LuRongSheng
Tutor: ZhengZheMin
School: Shaanxi Normal University
Course: Zoology
Keywords: Moth Moth Isoenzyme Electrophoresis Classification
CLC: S433
Type: Master's thesis
Year: 2000
Downloads: 141
Quote: 0
Read: Download Dissertation

Abstract

Currently, the classification and identification of the Lepidoptera, basically still use the traditional classification methods that were identified based on morphological characteristics of the external form and internal structure, but this approach often encounter some of the more difficult issues, such as the closely related species, sibling species identification. Traditional taxonomy the genus divided, taxonomists animal form, different traits to grasp and understand the results of the different types of vesting view. At this time the new classification means a comprehensive classification combined with traditional taxonomy, better able to solve these problems. In recent years, with the the isoenzyme understanding and research, the application of isozyme classification method can solve these problems. Isozyme is different from the structure in vivo, but the catalysis the same group of enzymes. Isozyme is composed of protein, while the protein synthesis is under genetic control, therefore, isozyme can reflect the nature of the genetic. The isozyme classification methods, the electrophoresis results obtained with the traditional classification results are basically the same, but also can distinguish between those who can not conclude that the type of traditional classification methods. Isoenzyme study phylogenetic kinship, genetic diversity and biological evolution degree is also a very useful side. In this paper, by polyacrylamide gel electrophoresis, some species of Shaanxi Province Lepidoptera Geometridae (Geomti-dae) and Arctiidae Arctiidae the EST isozymes electronic game analysis, the results are as follows. With individual EST isozyme comparative analysis of 11 species of the same kind of individual EST isozymes, the result is 11 individual the enzyme spectrum can be divided into four to five groups, it may be four to five loci of control. The same kind of individual zymography there are some differences, but the difference is relatively small, often there is a difference between a few individuals, most individuals data has basically the same zymography instructions in the same individual in some the allele higher frequency. Different developmental stages of Huai feet into the comparison of the four developmental stages and found Huai-foot locust at different stages of fat T isoenzyme there are some differences. Early number of bend insect enzyme with a relatively small, but the various stages of the enzymatic bands basically the same location EST isozymes during development is gradually expressed the full direction. In addition, through the the zymography comparison of each stage, also found Huai scale locust activity of different developmental stages, including late bend insects and pupae activity is the strongest, followed by adults, the weakest early bend worm activity. Three different body segments and gender comparison Wu moth different body segments EST isozymes comparison shows that, between different body segments EST isozymes is basically no difference, but the enzyme content of the abdomen is relatively large. Different between the sexes EST there are some differences, and the difference is greater than the difference between the same sex, this difference is still not sure of sex-linked. The results also show that the enzymatic activity of the enzyme activity of the female is often greater than the male. By the above three aspects can explain, when compared to that classification should be adopted in the same developmental stage, individuals of the same sex to compare. The four selenium isozymes free value of the three different species of the genus fq EST isozymes. Different species of the same genus asked EST isozymes there are some similarities and differences of more stable, the difference is mainly owned by some of the enzyme with another an, indicating that different species have different alleles. While the different colors of the type of enzyme to bring the higher frequencies. Therefore, the same enzyme esterase in two families of lower-order element classification. ; Moth' dissertation">Moth subfamily 5, 8 kinds of moss moth subfamily, Geometridae 16 Swimming power Rf value analysis, results are consistent with the traditional taxonomy.

Related Dissertations

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant pest and its control
© 2012 www.DissertationTopic.Net Mobile