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A Bioagent Formulation Of Metarhizium flavoviride And Its Control On Grasshopper

Author: YangBaoDong
Tutor: ZhaoKuiJun
School: Northeast Agricultural University
Course: Entomology
Keywords: Yellow Lvlv anisopliae Grasshoppers Virulence test Formulation screening Daejeon prevention
CLC: S433.2
Type: Master's thesis
Year: 2000
Downloads: 262
Quote: 2
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Abstract


In this study, in locust control at home and abroad on the basis of new Locusticide microbial agents - Metarhizium finish Formulation screening, role in the virulence of different kinds of locusts, field control effect and susceptible locusts tissue The pathological changes were studied. 1. Screened by two kinds of vegetable oil, three kinds of mineral oil twenty-two mixed into different proportions of mixed oil viscosity, evaporation rate, and the impact of Metarhizium anisopliae spore germination rate applicable to the steppe zone Metarhizium oil The agent formulation to determine the mixing ratio is 1:3, the peanut oil and diesel oil or kerosene mixed oil as the carrier oil of the preparation, but also filter out an ultraviolet absorber UV-1 UV-protecting agent as Metarhizium oil. 2. The finish determination of different kinds of locusts virulence of Metarhizium results: the Metarhizium anisopliae oil the Oedaleus locust indoor virulence role significantly higher than the locusts for trial, lethal concentration of 8.75 × 10 ~ 2 spores / ml processing 10d processing of various concentrations of locust adults of all deaths; Metarhizium oil for the trial of the remaining three grasshoppers (including the red wings wrinkle knee locust and drum wings wrinkle knee of adult locusts and the locust fourth instar nymphs) concentrations in the 14d after the death of more than 90% of the tested locusts. The Metarhizium anisopliae oil on the locust Oedaleus the most virulent of the drum-winged the wrinkled knees locust and the red wings wrinkle knee locust virulence second, relatively weak virulence of locust. Oedaleus asiaticus outdoor enveloped test results show that: concentration to 1.75 × 10 ~ 8 spores / ml in the handle 13d for the hoppers groups lethal rate reached 99.28%, while the concentration to 1.25 × 10 ~ 9 spores / ml in in the handle 14d death of the locusts adult groups only 78.4%. Test 1.25 × 10 ~ 9 spores / ml of the treatment can be used as a the adult field control test used at a concentration of 1.75 × 10 7 spores / ml concentration of oil is more suitable for hoppers Daejeon prevention, 3 to 4th instar grasshoppers best pest control state. 3. Indoor, outdoor virulence test results using Metarhizium oil Daejeon anti-efficiency test for two consecutive years in the grasslands, to obtain better control effect processing Daejeon 12d after sampling survey field control effect of 82.78%, treatment 37d field density decrease was still able to reach 89.71%. The results showed that: a high concentration (5 × 10 ~ 9 spores / ml) to achieve a satisfactory field control effect in the short term, the prevention and treatment as the treatment time at a low concentration (2.5 × 10 ~ 9 spores / ml) was no significant difference in the effect with a high concentration. To consider not only the choice of the best period of the field control locusts instars mixed population composition, population density and vegetation cover, but also consider the change of the weather conditions during field spraying. 4. This study on the basis of the virulence test, field trials, on susceptible locusts tissue pathological changes, electron microscopy, and the results showed that Metarhizium anisopliae conidia germination sprouting tubes for 24h after invasion of locusts in vivo to form of insect bacterial proliferation, absorb nutrients, host of adipose tissue cells late in the infection, formation of a large number of vacuoles eventually break.

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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant pest and its control > Orthoptera insects
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