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Genetic Analysis and RAPD Markers of Chipping Color in Diploid Potato (solanum tuberosum)

Author: JiangJie
Tutor: QuDongYu
School: Chinese Academy of Agricultural Sciences
Course: Olericulture
Keywords: Diploid potato Fried chip color Genetic analysis RAPD markers
CLC: S532.01
Type: Master's thesis
Year: 2000
Downloads: 109
Quote: 1
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Abstract


Fried potato chip processing industry faced a major problem is how to obtain a satisfactory fried chip color, study and solve this problem, the key is screening qualified raw materials. This paper systematically introduces fried potato chip color research the history and the main conclusions, fried potato chip color change mechanism and influencing factors, fried potato chip color three breeding strategies, fried potato chip color on the molecular biology of the latest progress. Fried slice of potato breeding colors, explore and use of wild species is very important to good genes. Identification of fried potato chip color new germplasm, analyzing their genetic composition, to explore the performance of fried chip color traits law helps to develop a reasonable breeding programs. Select the appropriate group for fried chip color marker genes for further study of molecular marker-assisted breeding and mapping and cloning genes related to deep-fried piece color foundation. In this study of three different combinations of diploid potato fried slice group materials, among populations on seven different processing characteristics were compared and analyzed, in accordance with certain procedures fried film processing plant for the three groups were all fried piece color identification, and analysis of hybrid groups (BSA) method was fried potato chip color gene RAPD markers. The main results are as follows: 1. According to the results of fried chip color identification, combined with other processing traits in group-based comprehensive comparative analysis of the results showed that GG is a good group of deep-fried pieces breeding material, which is expected to elect a number of good original diploid materials. 2. According to this test, fried piece color identification results and comparison with the two control varieties, based on the initial screening out 16 excellent fried chip color diploid plant, and then combined with other processing traits of a comprehensive assessment, the final screening out of nine integrated processing quality traits of the original diploid plant. 3. From three populations fried slice color value frequency distribution and the proportion of deep-fried pieces of color separation the following conclusions: three groups are not fried chip color values ??showed normal distribution, but rather presents a skewed distribution; fried potato chip color inherited from at least two or more of the gene. 4. Using RAPD markers, through more than 260 rigorous screening RAPD primers obtained two fried potato chip color gene in the RAPD markers OPH05 832 and OPW04 1268 .

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CLC: > Agricultural Sciences > Crop > Tuber Crops > Potatoes ( potatoes )
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