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Objective: To study the antimicrobial peptides Dermaseptin S4 (DS4), cecropin A-bombesin hybrid peptide (CA-MA) and Calliphoridae the antiviral peptide Alloferon1 (Alloferon1), in vitro, porcine reproductive and respiratory syndrome virus (PRRSV) and the Metro inhibition of disease virus (NDV), initially to explore its mechanism of action, and to provide a theoretical basis for the development of anti-PRRSV and NDV drugs. The PRRSV and NDV: Marc-145 and BHK-21 cell culture proliferation as the research object, using a different mode of administration (at the same time, infection after infection), the use of thiazole basket (MTT) assay cytopathic (CPE), measuring the absorbance value (D490nm) and half of the cells infective dose (TCID50), cell survival and PRRSV, NDV inhibition rate indicators to determine the antimicrobial peptides Dermaseptin S4, cecropin A-bombesin hybrid peptide and the Calliphoridae antiviral peptide Alloferon1 PRRSV, NDV inhibition strength. The results: 1. Of antimicrobial peptides Dermaseptin S4 vitro PRRSV inhibition, inhibition strength is proportional to its concentration, a significant difference (P lt; 0.01). DS4 PRRSV inhibitory effect of their mode of action, compared to three modes of action, DS4, most of the of PRRSV direct inactivation effect. 2. Dermaseptin S4 in vitro antimicrobial peptides NDV inhibited; compared to the three modes of action, directly inactivated the best, but not so obvious on the role of PRRSV. Cecropin A-bombesin the heterozygous peptide in vitro PRRSV inhibition, the inhibitory effect was nowhere near DS4 Alloferon1 and lamivudine control. Cecropin A-bombesin the heterozygous peptide in vitro NDV certain extent, compared with three modes of action, the CA-MA NDV directly inactivate better, but not so direct inactivation of PRRSV . NDV on the adsorption of the target cells, the intrusion of blocking and NDV the proliferation inhibition obvious in target cells. Calliphoridae anti the virus peptide Alloferon1 vitro of PRRSV inhibition, inhibition strength is proportional to its concentration. The three modes of action compared to direct inactivation effect of PRRSV, Alloferon1 best, less than the DS4. But Alloferon1 of PRRSV intrusion blocking the adsorption of the target cells and PRRSV stronger inhibitory effect on the value in the target cells the DS4 and CA-MA. Calliphoridae anti the virus peptide Alloferon1 vitro on NDV also inhibit the inhibitory effect was significantly higher than that the DS4 and CA-MA. Three modes of action compared, Alloferon1 NDV directly inactivated effect. Conclusion: 1. The antimicrobial peptides Dermaseptin S4, Calliphoridae anti the virus peptide Alloferon1 and cecropin A-bombesin hybrid peptide in vitro PRRSV and NDV have a certain degree of inhibition, inhibition of its concentration positively correlated. 2. The antimicrobial peptides Dermaseptin S4, the Calliphoridae antiviral peptide Alloferon1 and cecropin A-bombesin hybrid peptide in vitro inhibition of PRRSV and NDV its mode of action, which directly inactivated the best. 3 Preferably, the direct inactivation effect of the antimicrobial peptides Dermaseptin S4 vitro PRRSV NDV inhibition of proliferation in the target cells, the weakest. Calliphoridae anti the viral peptide Alloferon1 vitro direct inactivation effect on NDV preferably NDV inhibition of proliferation in the target cells, the weakest, but stronger than antimicrobial peptides Dermaseptin S4 and CA-MA. Activity of Calliphoridae antiviral peptide Alloferon1 in vitro anti-PRRSV and NDV best, cecropin A-bombesin hybrid peptide in vitro activity of the anti-PRRSV and NDV.
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