Dissertation > Excellent graduate degree dissertation topics show

Difference of Gene Expression in Wheat Roots under Fron- snffielent and Iron-deficient conditions and Cloning of some cDNA Fragments

Author: LiuWeiZhong
Tutor: QiuZeSheng；ZhangFuSuo
School: Capital Normal University
Course: Plant Physiology and Molecular Biology
Keywords: InRNA differential display Iron Deficiency Wheat Differential gene expression Iron stress inhibited Northern blot
CLC: Q945.78
Type: Master's thesis
Year: 2000
Downloads: 167
Quote: 3
Read: Download Dissertation

Abstract


By mRNA differential display (Differential Display Reverse Transcript PCR, referred DDRT-PCR) technology is relatively normal for iron and iron- deficiency stress -efficient wheat ( Beijing -411 ) and iron inefficient type wheat ( three genera Mai -3 ) of differences in gene expression . The results showed that both iron deficiency stress -induced expression of certain genes , can also inhibit the expression of some genes ; iron deficiency stress -efficient varieties ( Beijing -411 ) differences in gene expression than iron inefficient type wheat ( three genera Mai -3 ) significantly , indicating their ability to resist iron Deficiency indeed different. Pick out the use of differential display cDNA fragment as a probe the same from the iron -efficient wheat ( Beijing -411 ) in the normal stress treatment for iron deficiency apical and extracted total RNA by Northern hybridization , proved the difference fragment cDNA2 ( cloned then divided cDNA2-1 and cDNA2-2) and cDNA3 expression is regulated by iron deficiency stress suppressed. Sequencing and sequence analysis showed that , cDNA3 fragment encoding mitochondrial proteins with common wheat , and with the formation of the mitochondrial genes Cytc rpslA 99 % homology , and Arabidopsis plants with several dishes Cytc formation of the mitochondrial genes , 92 % or more homology ; initially identified after analysis of wheat rpslA gene expression in iron-deficiency stress weakened , Cytc formation decreased, affecting respiration and ATP generation . cDNA2-1 fragment with the ATP-binding-transporters have strong family resemblance , cDNA2-2 fragment with dDTP-4, 6 - glucose dehydratase has some homology , suggesting that these two genes in iron-deficiency stress may be weakened expression .

Related Dissertations

  1. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  2. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  3. The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
  4. Nitrogen Regulation and Management on High-yield Wheat-maize Rotation System,S512.1
  5. Effect of Nitrogenapplication Patterns on Variation of Soil Nitrate-N and Grain Yield of Wheat and Maize,S512.11
  6. Study on Nitrogen Accumulation Characteristics of Winter Wheat with Yield of 10t/ha, Diagnosis and Nitrogen Application Technologies,S512.1
  7. Optimization of Fermentation Conditions of Recombinant Lipoxygenase for the Production in Wheat Flour,TS201.25
  8. Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
  9. Characterization of Mineral Phosphate-Solubilizing Bacterium K3 and Colonization under Different Soil,S144.9
  10. Design and Implementation of the Growing Visualization System for Wheat Population,S512.1
  11. Trajectory Analysis of Wheat Aphids During Long-distance Migration Using HYSPLIT Model,S433
  12. Genetic Structure of the Wheat Powdery Mildew Populations from Henan, Hubei and Chongqing Province (City),S435.121
  13. Cloning 1Dx5 Gene from the Xinjiang Wheat Variety and Constructing Its Expression Vector,S512.1
  14. Studies on Regulating Effect of Nitrogen Rates on Starch Granule Distribution in Grain of Wheat,S512.1
  15. Accumulation of High Molecular Weight Glutenin Subunits in Wheat Grain as Regulated by Nitrogen Rate,S512.1
  16. Effects of Pre-Anthesis Drought Hardening on Grain Yield and Quality Formation in Wheat under Post-Anthesis Drought and Its Physiological Mechanism,S512.1
  17. Difference in Development between Culm and Tiller Roots and Their Contribution to Grain Yield and Quality in Wheat (Triticum Aestivum L.),S512.1
  18. Laws of Physiological Characteristics,dry Matter Accumulation and Transfer of Population After Anthesis in Two Kinds of Different Wheat Varieties,S512.1
  19. Molecular and Cytogenetic Identification of Triticum Aestivum-Leymus Racemosus Translocation Lines Involved in Leymus Racemosus Chromosome 7Lr,S512.1
  20. Identification and Molecular Mapping of a Novel Powdery Mildew Resistance Gene in Wheat,S512.1
  21. Effects of Environment on Wheat Quality and Combining Ability, Heterosis Research in Wheat,S512.1

CLC: > Biological Sciences > Botany > Plant Physiology > Hip forced Physiology
© 2012 www.DissertationTopic.Net  Mobile