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Preliminary Research and Establishment of National Standards of Classical Swine Fever Lapinised Vaccine

Author: LiJing
Tutor: LiuLi；WangZaiShi
School: Southwestern University
Course: Preventive Veterinary Medicine
Keywords: Classical swine fever attenuated vaccine National Standard Fluorescence quantitative RT-PCR
CLC: S852.5
Type: Master's thesis
Year: 2009
Downloads: 21
Quote: 0
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Abstract


Swine fever (Classical swine fever Vvirus, CSF) is a highly contagious disease caused by swine fever virus (Classical swine fever virus, CSFV), is classified by the World Organization for Animal Health (OIE) must be reported infectious diseases in animals. Currently, the outbreak of swine fever in China still is a pig the incidence up, endangering the largest and the most widespread infectious diseases. Prevention and treatment of these infectious diseases, classical swine fever vaccine or veterinary workers preferred. China's urgent need to develop in order to ensure the quality of the classical swine fever vaccine production stable, effective and controllable, disbursement and application of classical swine fever attenuated vaccine national standard. Attenuated Vaccine national standard products based on classical swine fever inspection of attenuated vaccine candidates after screening, analysis, determined the value of its standard features, developed a calibration for the quality of our classical swine fever vaccine than classical swine fever on national standards and control substances. The study established screening methods, screening a number of vaccine candidates, and determine the value of the relevant characteristics of the national standard of classical swine fever vaccine, will soon put into use during multi-party collaboration calibration, so not only can enhance the quality of domestic swine fever vaccine control, to improve the prevention and control of classical swine fever product quality, but also with international standards, and filling gaps in our lack of national standard of classical swine fever vaccine. This study, from raw materials, semi-finished products in strict accordance with the procedures of veterinary biological products require checks, screening of candidates after inspection by the manufacturers, to determine the characteristics of the standard value candidates developed the application of the new content of the method of valuation, and The new method is compared with traditional methods, given common values ??and mutual corroboration. Candidates using physical traits, loaded Determination, moisture determination, eight of the effectiveness of inspection, content inspection characteristic value assignment, \the establishment of a national standard developed procedures. The test will be introduced into the the National Vaccine standard developed TaqMan fluorescent quantitative RT-PCR method, the contrast with the traditional method to improve the preparation of a national standard. The results show that the method is sensitive up to 69 copies / μL, the linear range of detection of 10 ~ 2-10 ~ 7. CSFV national standard candidate positive detection rate reached 100% using this method Ct value is more stable, and good uniformity. Application of the research carried out on two batches of candidate the matter (83081225,2009011) initial screening results showed 2,009,011 batch of candidates physical traits pink spongy loose clumps, easy, and out of the bottle wall, quickly dissolved after adding diluent; installed Determination of weight, between 0.16 ~ 0.19g average of 0.1792g: CV 2.00%; moisture measurement results of water remaining volume ≤ 4%; effectiveness of test results for the the mature candidate effectiveness titer can reach diluted 1/450 times; the content Chau given the results were more than 8.35 × 10 ~ 5 copies / mL, the batch meets the requirements of the development of the national standard, as a candidate for multi-party collaboration calibration. The results of the application process, the new method and the conventional method can be mutually with the completion of the screening of candidate. In this study, both to complete the development of the national standard, they can standardize vaccine production, the new method can also be established at the same time in the development Auxiliary traditional rabbit body shaping thermal reaction is applied to the assessment of the classical swine fever vaccine potency, the pathogenesis of classical swine fever virus mechanism as well as the use of standard research.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Serological
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